During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology¿ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.

Focuses on human adipose-derived stem cells with additional coverage of ASCs of small and large animal species

Part I: Isolation Methods1. Isolation and Culture of Rhesus Adipose-Derived Stem CellsChristine Gagliardi and Bruce A. Bunnell2. Isolation of Human Adipose-Derived Stem Cells from LipoaspiratesGang Yu, Z. Elizabeth Floyd, Xiying Wu, Yuan-Di C. Halvorsen, and Jeffrey M. Gimble3. Isolation of Murine Adipose-Derived Stem CellsGang Yu, Xiying Wu, Gail Kilroy, Yuan-Di C. Halvorsen, Jeffrey M Gimble, and Z. Elizabeth Floyd4. In vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and DifferentiationMandi J. Lopez and Nakia D. Spencer5. In vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth CharacteristicsNakia D. Spencer and Mandi J. Lopez6. Adipogenic Differentiation of Adult Equine Mesenchymal Stromal CellsMartin A. Vidal and Mandi J. Lopez7. Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem CellsKellie J. Williams, Robert A. Godke, and Kenneth R. Bondioli8. Automated Isolation and Processing of Adipose-Derived Stem and Regenerative CellsKevin C. Hicok and Marc H. HedrickPart II: Phenotyping Methods9. Methods for the Purification and Characterization of Human Adipose-Derived Stem CellsStan Gronthos and Andrew C.W. Zannettino10. Characterization of Human ASCs Using Flow CytometryH. Alan Tucker and Bruce A. Bunnell11. Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal CellsKevin R. McIntosh12. Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell ContaminantsPauline Decaunes, David Estève, Alexia Zakaroff-Girard, Coralie Sengenès, Jean Galitzky, and Anne Bouloumié13. Gel-Based and Gel-Free Proteomic TechnologiesPeter Scherp, Ginger Ku, Liana Coleman, and Indu KheterpalPart III: Differentiation Methods: In vitro and In vivo Applications14. Adipogenic Differentiation of Adipose-Derived Stem CellsGang Yu, Z. Elizabeth Floyd, Xiying Wu, Teddi Hebert, Yuan Di C. Halvorsen, Ben Buehrer, and Jeffrey M. Gimble15. 3D Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem CellsBradley T. Estes and Farshid Guilak16. Neural Differentiation of Human ASCsJi Min Yu, Bruce A. Bunnell, and Soo-Kyung Kang17. Osteogenic Differentiation Strategies for Adipose-Derived Mesenchymal Stem CellsRobert Jan Kroeze, Marlene Knippenberg, and Marco N. Helder18. Generation of Adipose Stromal Cell-Derived Hepatic CellsJoseph C. Ruiz19. Adipose Stem Cell Differentiation into Smooth Muscle CellsKacey G. Marra, Candace A. Brayfield, and J. Peter Rubin20. Endothelial and Cardiac Regeneration from Adipose TissuesLouis Casteilla, Valérie Planat-Bénard, Stéphanie Dehez, Sandra De Barros, Corinne Barreau, and Mireille André21. Epithelial Differentiation of Human Adipose-Derived Stem CellsPatrick C. Baer, Martin Brzoska, and Helmut Geiger22. Screening for Epigenetic Target Genes that Enhance Reprogramming Using Lentiviral Delivered shRNAJong S. Rim, Karen Strickler, Christian Barnes, Lettie Harkins, Jaroslaw Staszkiewicz, Rachel Power, and Kenneth J. EilertsenPart IV: Bioengineering, Manufacturing, and Clinical Technologies23. Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk ScaffoldsJennifer H. Choi, Evangelia Bellas, Gordana Vunjak-Novakovic, and David L. Kaplan24. A First Approach for the Production of Human Adipose Tissue-Derived Stromal Cells for Therapeutic UsePhilippe Bourin, Julie-Anne Peyrafitte, and Sandrine Fleury-Cappellesso25. Viral Transduction of Adipose-Derived Stem CellsPatricia A. Zuk26. Use of Adipose-Derived Stem Cells in High Throughput Screening to Identify Modulators of LipogenesisY. Reneé Lea-Currie, David J. Duffin, and Benjamin M. Buehrer27. Preservation Protocols For Human Adipose Tissue-Derived Adult Stem CellsRam Devireddy and Sreedhar Thirumala28. Soft Tissue ReconstructionJ. Peter Rubin and Kacey G. Marra29. Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem CellsYeon Jeong Kim and Jin Sup Jung30. The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem CellsBrigitte Wdziekonski, Tala Mohsen-Kanson, Phi Villageois, and Christian Dani31. Cell Sheet Technology for Tissue Engineering: The Self-Assembly Approach Using Adipose-Derived Stromal CellsBenoît Labbé, Guillaume Marceau-Fortier, and Julie Fradette32. Differentiation of Adipose-Derived Stem Cells for Tendon RepairA. Cagri Uysal and Hiroshi Mizuno33. Adipose-Derived Stem Cells for Skin RegenerationHiroshi Mizuno and Masaki Nambu34. Adipose-Derived Stem Cells for Periodontal Tissue RegenerationMorikuni Tobita and Hiroshi Mizuno