Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

Contains a plethora of modern adaptations to the FISH methodology

Part I: Core Techniques1. Fluorescence in situ Hybridization (FISH), Basic Principles, and MethodologyElisa Garimberti and Sabrina Tosi2. Fluorescence in situ Hybridization on DNA Halo Preparations and Extended Chromatin FibersLauren S. Elcock and Joanna M. Bridger3. Detection of Nascent RNA Transcripts by Fluorescence in situ HybridizationJill M. Brown and Veronica J. Buckle4. Fluorescence in situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue MicroarraysBrenda M. Summersgill and Janet M. Shipley5. Fluorescence in situ Hybridization (FISH) on Tissue CryosectionsIrina Solovei6. Multiplex Fluorescence in situ Hybridization (M-FISH)Rhona Anderson7. Optical Mapping of Protein-DNA Complexes on Chromatin FibersBeth Sullivan8. 3D-FISH on Cultured Cells Combined with ImmunostainingIrina Solovei and Marion CremerPart II: Technical Advancements and Novel Adaptations9. The Comet-FISH Assay for the Analysis of DNA Damage and RepairGraciela Spivak10. Direct in situ Hybridization with Oligonucleotide Functionalized Quantum Dot ProbesLaurent A. Bentolila11. LNA-FISH for Detection of microRNAs in Frozen SectionsAsli N. Silahtaroglu12. Chromosome Orientation Fluorescence in situ Hybridization (CO-FISH) or Strand-Specific FISHSusan M. Bailey, Eli S. Williams, Michael N. Cornforth, and Edwin H. Goodwin13. Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live CellsEberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stei, Carmen Boxler, Patrick Müller, Andriy Mokhir, Roland Krämer, Christoph Cremer, and Michael Hausmann14. Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA DenaturationDaniela Moralli and Zoia L. Monaco15. CryoFISH: Fluorescence in situ Hybridization on Ultrathin CryosectionsSheila Q. Xie, Liron-Mark Lavitas, and Ana Pombo16. Characterization ofChromosomal Rearrangements Using Multicolor-Banding (MCB/m-band)Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, and Nadezda Kosyakova17. Visualizing Nucleic Acids in Living Cells by Fluorescence in vivo HybridizationJoop Wiegant, Anneke K. Brouwer, Hans J. Tanke, and Roeland W. DirksPart III: Translational FISH: Applications for Human Genetics and Medicine18. Quality Control in FISH as Part of a Laboratory¿s Quality Management SystemRos Hastings19. FlashFISH: ¿Same Day¿ Prenatal Diagnosis of Common Chromosomal AneuploidiesSherry Ho and Mahesh A. Choolani20. FISH for Pre-Implantation Genetic DiagnosisPaul N. Scriven and Caroline Mackie Ogilvie21. PNA-FISH on Human SpermFranck Pellestor, Cécile Monzo, and Samir Hamamah22. POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation PolymorphismAnja Weise, Madeleine Gross, Sophie Hinreiner, Vera Witthuhn, Hasmik Mkrtchyan, and Thomas Liehr23. Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH)Agata Kowalska, Eva Bozsaky, and Peter F. Ambros24. ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy MaterialSoo-Yong Tan and Goran Mattsson25. Fluorescence in situ Hybridization on 3D Cultures of Tumor CellsKaren J. Meaburn26. Simultaneous Ultrasensitive Subpopulation Staining/Hybridization in situ (SUSHI) in HIV-1 Disease MonitoringBruce PattersonPart IV: Protocols for Model Organisms27. Detection of Prokaryotic Cells with Fluorescence in situ HybridizationKatrin Zwirglmaier28. FISH as a Tool to Investigate Chromosome Behavior in Budding YeastHarry Scherthan and Josef Loidl29. FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrataEdwin C. Odoemelam, Nithya Raghavan, Wannaporn Ittiprasert, Andre Miller, Joanna M. Bridger, and Matty Knight30. Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic