.1321, Methods in Molecular Biology
Methods and Protocols
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Features protocols in glyco-engineering in bacteria, yeast, insects, plants and mammalian cells
MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

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Autor: Alexandra Castilho
ISBN-13 :: 9781493949427
ISBN: 149394942X
Verlag: Springer, Berlin, Springer New York, Humana Press
Seiten: 439
Sprache: Englisch
Auflage Softcover reprint of the original 1st ed. 2015
Sonstiges: Taschenbuch, 235x155x mm, 27 SW-Abb., 52 Farbabb., 13 Tabellen
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