Thissecond edition details new emerging areas of zebrafish research focusing on genetics
and genomics, techniques for developing and analyzing zebrafish disease models,
and methods for neuroscience. Zebrafish:
Methods and Protocols, Second Edition guides readers through methods for mutagenesis
and genome editing in zebrafish, applications
of GFP-expressing transgenic fish, techniques for cancer models, imaging of infection
and host-pathogen interactions, metabolism and transport of lipids, and the structure and function of neural circuits
and their role in generating behavior. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.

Chemical Screening in Zebrafish.- TALEN-Mediated
Mutagenesis and Genome
Editing.- Homology
Independent Integration of Plasmid DNA into the Zebrafish Genome.- Detection
of Multiple Genome Modifications Induced by the CRISPR/Cas9 System.- Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish.- Testing of cis-Regulatory Elements by Targeted Transgene Integration in
Zebrafish using PhiC31 Integrase.- Fluorescence-Activated Cell Sorting and Gene Expression Profiling
of GFP Positive Cells from Transgenic Zebrafish Lines.- Construction of the Inbred Strain.- Zebrafish as a Model for the
Study of Solid Malignancies.- Melanoma Regression and Recurrence in Zebrafish.- Imaging of Human Cancer Cell Proliferation, Invasion and
Micrometastasis in a Zebrafish Xenogeneic Engraftment Model.- Modeling Leukemogenesis in the Zebrafish using Genetic
and Xenograft Models.- Enumerating
Hematopoietic Stem and Progenitor Cells in Zebrafish Embryos.- Live Imaging of Host-Pathogen
Interactions in Zebrafish Larvae.- Methodologies
for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish.- Studying Lipid Metabolism and Transport During Zebrafish
Development.- Targeted Electroporation in Embryonic, Larval and Adult Zebrafish.- Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy.- In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain.- Quantifying Aggressive Behaviour in Zebrafish.- Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish.- A Practical Guide to Light Sheet Microscopy.- Calcium Imaging of Neuronal
Activity in Free-Swimming Larval Zebrafish.- Fiber Optic-Based Photostimulation of Larval Zebrafish.- Genetic Ablation, Sensitization and Isolation of Neurons Using Nitroreductase
and Tetrodotoxin-Insensitive Channels.