This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer¿s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagentsreadily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

Includes cutting-edge methods and protocols for the study of recombinant integral membrane

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functionaland Structural Studies.- Membrane Protein Production in the Yeast, S. cerevisiae.- Membrane ProteinsProduction in Escherichia coli:Protocols and Rules.- Codon Optimizing for Increased Membrane ProteinProduction: A Minimalist Approach.- Generation of Tetracycline-InducibleMammalian Cell Lines by Flow Cytometry for Improved Overproduction of MembraneProteins.- Membrane Protein Production in Lactococcus lactis for FunctionalStudies.- Expression ofViral Envelope Glycoproteins in Drosophila melanogaster S2 Cells.- Leishmania tarentolæ as a Promising Toolfor Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic MembraneProteins: The Case of the ABC Pump ABCG6.- Overexpression, MembranePreparation, and Purification of a Typical Multidrug ABC Transporter BmrA.- Expression ofEukaryotic Membrane Proteins in Pichiapastoris.- Integral Membrane Protein Expression in Saccharomyces cerevisiae.- High-Throughput Baculovirus ExpressionSystem for Membrane Protein Production.- Small-Scale Screening to Large-ScaleOver-Expression of Human Membrane Proteins for Structural Studies.- Purificationof Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies.- MembraneProtein Solubilization and Composition of Protein Detergent Complexes.- Detergent-FreeMembrane Protein Purification.- Conformational Dynamics and Interactions ofMembrane Proteins by Hydrogen/Deuterium Mass Spectrometry.- Lessons from an a-Helical Membrane Enzyme: ExpressionPurification, and Detergent Optimization for Biophysical and StructuralCharacterization.- Method to Screen Multidrug Transport Inhibitors Using YeastOver-Expressing a Human MDR Transporter.