Thorough and practical, Human Embryonic Stem Cell Protocols, Third Edition serves as a valuable resource to all those interested in exploring stem cell biology questions in a research setting.

Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Blastocysts.- Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel.- Growth of Human Pluripotent Stem Cells Using Functional Human Extracellular Matrix.- Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate.- Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells.- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR.- Study of Gap Junctions in Human Embryonic Stem Cells.- Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions.- Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells.- Genetic Manipulation of Human Embryonic Stem Cells.- Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System.- Use of Multicolor Flow Cytometry for Isolation of Specific Cell Populations Deriving from Differentiated Human Embryonic Stem Cells.- Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation.- Derivation of Endothelial Cells and Pericytes from Human Pluripotent Stem Cells.- Differentiation of Human Embryonic Stem Cells on Periodontal Ligament Fibroblasts.- Derivation of Epithelial Cells from Human Embryonic Stem Cells as an In Vitro Model of Vocal Mucosa.- Human Embryonic and Hepatic Stem Cell Differentiation Visualized in Two and Three Dimensions Based on Serial Sections.- Derivation of Chondrogenic Cells from Human Embryonic Stem Cells for Cartilage Tissue Engineering.- Microgrooved Surface Modulates Neuron Differentiation in Human Embryonic Stem Cells.- Directed Differentiation of HumanEmbryonic Stem Cells into Neural Progenitors.- Direct Conversion of Pluripotent Human Embryonic Stem Cells Under Defined Culture Conditions into Human Neuronal or Cardiomyocyte Cell Therapy Derivatives.- Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small Molecule Inhibitor of Src Family Kinases.- Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells.- Accelerated Three-Dimensional Neuroepithelium Formation from Human Embryonic Stem Cells and Its Use for Quantitative Differentiation to Human Retinal Pigment Epithelium.- Efficient Production of Photoreceptor Precursor Cells from Human Embryonic Stem Cells.- Generation of Megakaryocytes and Platelets from Human Pluripotent Stem Cells.- A Simple Protocol for the Generation of Cardiomyocytes from Human Pluripotent Stem Cells.