Insoluble Proteins

Methods and Protocols
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Includes cutting-edge methods and protocols involving insoluble proteins
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1. General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins
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With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

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Autor: Elena García-Fruitós
ISBN-13 :: 9781493922048
ISBN: 1493922041
Erscheinungsjahr: 02.12.2014
Verlag: Springer New York
Gewicht: 1006g
Seiten: 444
Sprache: Englisch
Auflage 2015
Sonstiges: Buch, 261x184x32 mm
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