In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.

Includes cutting-edge methods and protocols

Fixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae.- MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos.- Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies.- Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian.- In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae.- LNA-based in Situ Hybridization Detection of mRNAs in Embryos.- Chromogen Detection of microRNA in Frozen Clinical Tissue Samples using LNA(TM) Probe Technology.- MicroRNA In Situ Hybridisation in Tissue Microarrays.- Fluorescence in situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections.- Sensitive and Specific in situ Hybridisation for Early Drug Discovery.- Zinc Based Fixation for High Sensitivity in situ Hybridization: A Non-Radioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections.- Dual-color Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope.- Fully Automated Fluorescence-based 4-color Multiplex Assay for Co-detection of microRNA and Protein Biomarkers in Clinical Tissue Specimens.- Multiplexed miRNA Fluorescence in situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues.- Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris® RNA Fluorescence in situ Hybridization in Adherent Cells.- Quantitative Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope.- Identification of Low Expressing Transcripts of the NPY Receptors' Family in the Murine Lingual Epithelia.- In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes.- In situ Hybridization Freeze-assisted Punches (IFAP): Technique for Liquid-based TissueExtraction from Thin Slide-mounted Sections for DNA Methylation Analysis.- miRNA Detection at Single Cell Resolution Using Microfluidic LNA flow-FISH.- PNA-based Fluorescence in situ Hybridization for Identification of Bacteria in Clinical Samples.