Practical and authoritative, Herpes Simplex Virus: Methods and Protocols will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques.

HSV-1 Biology and Life Cycle.- Herpes Simplex Virus Growth, Preparation, and Assay.- Isolation of Herpes Simplex Virus Nucleocapsid DNA.- Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes.- Engineering HSV-1 Vectors for Gene Therapy.- Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors.- HSV-1 Amplicon Vectors as Genetic Vaccines.- Modification of HSV-1 to an Oncolytic Virus.- Herpes Simplex Virus Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo.- Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals.- Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation.- Characterization of Extracellular HSV-1 Virions by Proteomics.- A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors.- Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein-Protein Interactions.- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy.- Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication.- Expression, Purification and Crystallization of HSV-1 Glycoproteins for Structure Determination.- Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components.- In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells.- HSV-1 Protein Expression Using Recombinant Baculoviruses.- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.