Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.

Contributors to Volume 3PrefaceVolumes in SeriesSection I Autoradiography 1. Autoradiographic and Microchemical Methods for Quantitation of Steroid Receptors 2. High-Resolution Autoradiographic Mapping of Drug and Hormone Receptors 3. High-Resolution Autoradiographic Imaging of Brain Activity Patterns with Radiolabeled 2-Deoxyglucose and Glucose 4. Double- and Single-Label Quantitative Autoradiography for Cerebral Physiology 5. Combination of Tritiated Thymidine Autoradiography and Neuropeptide Immunocytochemistry to Determine Birthdates and Migration Routes of Luteinizing Hormone-Releasing Hormone NeuronsSection II Quantification of Structures: Statistical and Computational Methods 6. Techniques and Technology for Dynamic Video Imaging of Cellular Fluorescence 7. Three-Dimensional Computer Reconstruction of Perforated Synapses 8. Determination of Numerical Density of Perforated and Nonperforated Synapses 9. Efficient and Unbiased Sampling of Nerve Fibers for Estimating Fiber Number and Size 10. Methods for Analyzing Neuronal Connections in Mammals 11. Image Analytic Techniques for Quantification of Immunohistochemical Staining in the Nervous System 12. Methods for Visualizing and Analyzing Individual Axon ArborsSection III Tracing Neuronal Connections: Tracers, Toxins, and Dyes 13. Phaseolus vulgaris Leucoagglutinin Anterograde Axonal Transport Technique 14. Retrograde Axoplasmic Transport of Neurotoxins 15. Tracing Neuronal Connections in the Periphery: Renal Nerves 16. Small Injections of Axonally Transported Fluorescent TracersSection IV Staining Technology 17. Fluoro-Gold and 4-Acetamido-4'-isothiocyanostilbene-2,2,-disulfonic Acid: Use of Substituted Stilbenes in Neuroanatomical Studies 18. Silver Impregnation Method for Frozen Sections of Human Nervous Tissue Using Ammoniacal Silver-Dichromate Solution 19. Silver Impregnation Method for Neurons Using Synthetic Surfactants: A Contribution to Golgi MethodSection V Freezing Techniques 20. Use of Freeze-Fracture in Neurobiology 21. Cryoprotection and Freezing Methods to Control Ice Crystal Artifact in Frozen Sections of Fixed and Unfixed Brain TissueSection VI Combined and High-Resolution Methods 22. Double-Label [3H]2-Deoxyglucose and [14C]2-Deoxyglucose Method for Mapping Brain Activity Underlying Two Experimental Conditions in the Same Animal 23. Application of Incident Light Polarization Microscopy 24. Light Microscopic Localization of Drug and Neurotransmitter Receptors in the Brain 25. Light and Electron Microscopic Investigation of Somatostatin-Containing Neurons in the Central Nervous System 26. Reduced Nicotinamide Adenine Dinucleotide Phosphate-Diaphorase Histochemistry: Light and Electron Microscopic InvestigationsAddendum Addendum to Article [6]Index