Methods of Animal Experimentation, Volume I, provides information on the most common methods for using animals as tools in the search for new biological knowledge. The techniques described will facilitate the most efficient use of research animals and provide guidelines for their utmost comfort and welfare. The text is arranged according to specific research methods rather than to organ system or disease category. This approach gives the reader a broad view of the techniques involved in specific fields and describes the range of usefulness of these techniques. The first five chapters of the present volume describe basic information, methods, and principles involved in managing animals for experimental procedures. The remaining chapters deal with special techniques which have been demonstrated to be distinct, useful methods for using laboratory animals as a basic biomedical research tool. The descriptions of both fundamental and well-developed techniques of animal experimentation in various research fields should be useful to graduate students and experienced scientists who must consider variations in research approaches. The book is a source of information for the scientist administrator who is frequently confronted with different proposed approaches to biological research projects utilizing animals.

List of ContributorsPrefaceContents of Volume II IntroductionChapter 1 Collection and Withdrawal of Body Fluids and Infusion Techniques I. Introduction II. Collection and Withdrawal of Body Fluids III. Infusion Techniques ReferencesChapter 2 Anesthesia and Sedation I. Introduction II. Aims of an Ideal General Anesthetic III. Objectives and Purposes of Different Types of Anesthesia IV. Basic Principles V. Methods of Preanesthesia VI. Methods of Anesthesia VII. The Use of Postoperative Analgesics and Sedatives VIII. Emergency Measures for Experimental Animals Undergoing Procedures Requiring an Anesthetic Agent IX. Preparation of Anesthesia of Individual Experimental Animals X. Concluding Comments ReferencesChapter 3 Care of Animals During Surgical Experiments I. Introduction II. Preoperative Care III. Care During Operation IV. Postoperative Care ReferencesChapter 4 Radiography I. Introduction II. Principles of Radiographic Interpretation III. Principles of Radiographic Technique IV. Radiation Safety V. Conclusion References Additional BibliographyChapter 5 Methods of Euthanasia and Disposal of Laboratory Animals I. Introduction II. Factors Involved in Euthanasia III. Reasons for Euthanasia IV. Procedures V. Disposal VI. Source Material ReferencesChapter 6 Methods of Parasitic Infections: Outline of General Principles I. Introduction II. Cautions in the Choice of a Parasite and Animal Host III. Factors Which Influence the Course of Infection with a Parasite ReferencesChapter 7 Methods in Germfree Animal Research I. Introduction II. Germfree Animal Equipment III. Securing and Maintaining Germfree Animals IV. The Germfree Animal on Experiment V. Some Information Derived from Germfree Animal Studies VI. Outlook for the Future ReferencesChapter 8 Aerosol Challenge of Animals I. Introduction II. Basic Aerosol Terminology III. Aerosol Test Facilities and Systems IV. Generation of Experimental Aerosols V. Sampling Biological Aerosols VI. Aerosol Challenge of Animals VII. Maintenance of Aerosol-Challenged Animals VIII. Diagnostic Procedures for Aerosol-Challenged Animals IX. Laboratory Design Principles X. Safety Management in Infectious Disease Laboratories ReferencesChapter 9 Principles in Drug Administration I. Introduction II. Preparation of Animal III. Preparation of Drug IV. Gastrointestinal Routes V. Parenteral Routes VI. Inhalation Route VII. Topical Route VIII. Choice of Animal Species IX. Experimental Design and Statistical Evaluation X. Conclusion References Supplementary BibliographyAuthor IndexSubject Index