Methods in Neurosciences, Volume 13: Neuropeptide Analogs, Conjugates, and Fragments covers the synthesis and characterization of peptide analogs, conjugates, and fragments, their use as ligands for receptors, and their role in the development and use of antisera. The book discusses techniques such as novel synthetic approaches; biotinylation; purification and characterization; radioligand techniques and assay development; use of agonists and antagonists; distinguishing receptor subtypes; conjugation to carrier proteins; antiidiotypic antibody development; and radiolabeling. Neuroscientists, biochemists, neurochemists, and pharmacologists will find the book useful.

Contributors to Volume 13Preface Volumes in Series Section I Synthesis and Characterization 1. Synthetic Approaches to Incorporation of Novel Amino Acids into Gonadotropin-Releasing Hormone Peptides 2. Synthesis of Pseudopeptides 3. Synthesis and Synthetic Manipulations of Peptides Derived from Parathyroid Hormone and Parathyroid Hormone-Related Protein 4. Biotinylated Neuropeptide Analogs: Design and Use as Probes for Target Cells in Heterogeneous Populations 5. Peptide Characterization and Purification Using High-Performance Liquid ChromatographySection II Development and Use of Receptor Ligands 6. Atrial Natriuretic Factors and Fragments 7. Atrial Natriuretic Factor Binding to Clearance Receptors in Isolated Neuronal Membranes 8. Biotinylated Endothelin Analogs as Probes for Endothelin Receptor 9. Analogs of Endothelin 10. Selective Cholecystokinin A and Cholecystokinin B/Gastrin Receptor Agonists 11. Tachykinin Receptor Antagonists 12. Somatostatin Receptor Subtypes in Rat Brain Revealed by Somatostatin Analogs 13. Thyrotropin-Releasing Hormone: Analogs and Receptors 14. Distinguishing Bombesin Receptor Subtypes 15. Bombesin Receptor Subtypes: Characterization and Solubilization 16. Nonpeptide Angiotensin II Receptor Antagonist: Losartan 17. Via Vasopressin Receptors: Selective Biotinylated Probes 18. Via Vasopressin Receptors: Studies with Radioiodinated Ligand 19. Pharmacological Identification of Vasopressin Receptors in Isolated Renal TubuleSection III Development and Use of Antisera 20. Neuropeptide Conjugation to Carrier Protein 21. Antiidiotypic Thyrotropin-Releasing Hormone Antibody 22. 125I-Labeled Substance P: Influence of Oxidation State on Sensitivity of RadioimmunoassayIndex