Current Topics in Cellular Regulation, Volume 13 emphasizes the basic mechanisms involved in the regulation of diverse cellular activities.This book discusses the enzymology of aminoacyl-tRNA protein transferases, analysis of mutant phenotypes, and protein kinase reaction with pyruvate kinase as substrate. The rapid hormonal regulation of pyruvate kinase in vivo, interconvertible enzyme cascades in metabolic regulation, monocyclic cascade systems, and metabolism of fructose in the liver are also elaborated. This publication likewise covers the influence of fructose on lipid metabolism, lactose operator-repressor interaction, and regulation of nitrogenase synthesis. Other topics include the involvement of isozymes in metabolic regulation and molecular approach to the complement system.This volume is a good reference for biologists and researchers conducting work on the advances in the general area of cellular regulation.

ContentsList of ContributorsPosttranslational NH2-Terminal Aminoacylation I. Introduction II. Enzymology of Aminoacyl-tRNA Protein Transferases III. Analysis of Acceptor Proteins IV. Analysis of Mutant Phenotypes V. Discussion Note Added in Proof ReferencesThe Regulation of Liver Pyruvate Kinase by Phosphorylation-Dephosphorylation I. Introduction II. Purification and Some Properties of Liver Pyruvate Kinase III. Phosphorylation of Liver Pyruvate Kinase in Vitro and Its Effect on the Kinetic Properties of the Enzyme IV. Phosphorylated Site of Pig Liver Pyruvate Kinase. Subtilisin-Catalyzed Modification V. The Protein Kinase Reaction with Pyruvate Kinase as Substrate VI. Phosphorylation of Peptides with Structures Related to the Phosphorylatable Site of Pyruvate Kinase VII. Dephosphorylation of Pyruvate Kinase with Phosphoprotein Phosphatase VIII. Rapid Hormonal Regulation of Pyruvate Kinase in Vivo IX. Conclusions ReferencesInterconvertible Enzyme Cascades in Metabolic Regulation I. Introduction II. Monocyclic Cascade Systems III. Bicyclic Cascade Systems IV. Multicyclic Cascade Systems V. Discussion ReferencesMetabolic Effects of Fructose in the Liver I. Introduction II. Metabolism of Fructose in the Liver III. Influence of Fructose on the Metabolism of Glycogen IV. Effect of Fructose on the Catabolism of Purine Nucleotides V. Influence of Fructose on Lipid Metabolism VI. Effect of Fructose on Alcohol Metabolism VII. Concluding Remarks ReferencesLactose Operator-Repressor Interaction I. Introduction II. The Lac Repressor III. The Lac Operator IV. Lactose Operator-Repressor Interaction V. Models for the Operator-Repressor Interaction and Concluding Remarks ReferencesRegulation of Nitrogen Fixation I. Introduction II. Regulation of Nitrogenase Activity III. Regulation of Nitrogenase Synthesis IV Electron Transport and ATP Production Coupled to N2 Reduction V. Other Related Regulation VI. Synopsis ReferencesThe Role of Isozymes in Metabolism: A Model of Metabolic Pathways as the Basis for the Biological Role of Isozymes I. Introduction II. The Involvement of Isozymes in Metabolic Regulation III. The Role of Compartmentation in Metabolic Regulation IV. A Hypothetical Model of Metabolism: Polyisozymic Complexes V. Polyisozymic Complexes in Glucose Utilization VI. Suggested Experimental Tests of the Hypothesis VII. Concluding Remarks ReferencesA Molecular Approach to the Complement System I. Introduction II. The Complement System III. Individual Components IV. Mechanisms of Action V. Comparisons of Structure and Function VI. Conclusions and Comments ReferencesSubject IndexContents of Previous Volumes