This book provides an integrated description of methods used to rear vectors of human, higher animal, and plant pathogens in the laboratory. It deals with diverse subject areas, and contains descriptions of standard, as well as highly specialized, methods used by medical, veterinary, entomology, and plant pathology experts. The text brings together the standard breeding and manipulation methods developed in America, Europe, Asia, and Africa. It describes the cultivating, handling, sterile techniques, and cell culture as well as safety measures to prevent contamination and escape of insects, ticks, nematodes and fungal vectors.

Preface. Laboratory Maintenance of Mosquitoes. Black Fly Rearing and Use in Laboratory Bioassays. Maintenance of Ticks in the Laboratory. Maintenance of the Triatomine Bugs Rhodnius prolixus and Triatoma dimidiata under Laboratory Conditions. Laboratory Rearing of Bed Bugs. Laboratory Maintenance of Phlebotomine Sand Flies. Establishment and Maintenance of Small Scale Tsetse Colonies. Rearing Aphids to use in Virus-Vectors Studies. Leafhopper and Planthopper Rearing. Maintenance of Piesma quadratum (Fieb.). Rearing Thrips Vectors. Whiteflies as Subjects of Laboratory Research. Xiphinema index, Maintenance and Feeding in Monoxenic Cultures. Xiphinema index, Rearing in Greenhouse. Maintenance of Fungal Vectors of Plant Viruses. Sterile Techniques for Rearing and Cell Culture of Leafhopper Vectors of Plant Pathogens. Psyllid Vectors of Plant Pathogens: Collection, Rearing and Use in Laboratory Research Studies. Index.