The focus of this symposium was on the present and future capabilities of flow cytometry for both medical and biological applications in cancer. This technology began with quite modest instrumentation, with limited capabilities to answer biological questions. Today, both the clinical workhorses and the powerful multi-laser, multi-detector, sorting machinery, coupled with sophisticated computers and storage devices and the increasing storehouse of markers and dyes, are taking us to the limit and beyond in finding answers to the cause and cure of cancer.In the past, both normal hematopoietic tissue and leukemias have been the tissue samples of choice in the application of flow cytometry, and some of the most recent applications with these tissues are presented here. However, the book also discusses the increasingly sophisticated disaggregation techniques which allow investigators the possibility to train their lasers on solid tumors. Not only can we use flow cytometry with associated fluorescent markers to understand the biology of cancer, but also the wide array of existing and developing markers provides us with important diagnostic tools in the detection of cancer early in either the malignant or relapse process. And the field comes full circle, with the use of the technology for gene mapping and other genetic studies to unlock the basic malignant process.

1. Cytometry 2000: Laser Probing the Future.- 2. Activation and Proliferation of Purified Hemato-Poietic Precursor Cells from Human Bone Marrow.- 3. Flow Cytometric Proliferative Fraction Analysis in Solid Tumors.- 4. Mitogenic and Non-Mitogenic Induction of Lympho-Cytic Invasion: Dual Parameter flow Cytometric Analysis.- 5. Flow Cytometric Monitoring of Drug Resistance in Human Solid Tumors.- 6. Flow Cytometry in Oncology: Some Lessons from History.- 7. DNA Flow Cytometry Application to Clinical Trials in Breast Cancer.- 8. Processing of Solid Tumors for DNA analysis by Flow Cytometry.- 9. SJL Model for Lymphoma Treatment.- 10. Gene Mapping and Molecular Cytogenetics Using Flow-Sorted Chromosomes.- 11. The Clinical Potential of DNA Content Parameters in Human Pediatric and Adult Solid Tumors.- 12. Differentiation of Human B-Cell Tumors: A Preclinical Model for Differentiation Therapy.- 13. Probing Immune Responses: The Role of Intra-Cellular Glutathione.- 14. DNA Topoisomerase II Poisons and the Cell Cycle.- 15. In Situ Hybridization of Centromeric DNA as Visualized on the Nuclear Matrix by Laser Confocal Cytometry.- 16. Megakaryocytopoiesis 2000.- 17. The Molecular Basis for the Control of Mammalian Cell growth.- 18. Isolation of Rare Cells by High-Speed Flow Cytometry and High-Resolution Cell Sorting for Subsequent Molecular Characterization - Application in Prenatal Diagnosis, Breast Cancer and Autologous Bone Marrow Transplantation.