The book is structured in five sections, each containing several chapters written by experts and major contributors to particular topics. The volume starts with a historical perspective and fundamental principles of membrane potential imaging and continues to cover the measurement of membrane potential signals from dendrites and axons of individual neurons, measurements of the activity of many neurons with single cell resolution, monitoring of population signals from the nervous system, and concludes with the overview of new approaches to voltage-imaging. The book is targeted at all scientists interested in this mature but also rapidly expanding imaging approach.

Outlines methods and procedures in imaging cellular activity in neurons using voltage-sensitive dyes 2.

Chapter 1: Historical overview and general methods of membrane potential imagingLawrence B CohenChapter 2: Design and use of organic voltage sensitive dyesLeslie M LoewChapter 3: Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spinesMarco Canepari, Marko Popovic, Kaspar Vogt, Knut Holthoff, Arthur Konnerth, Brian M Salzberg, Amiram Grinvald, Srdjan D Antic and Dejan ZecevicChapter 4: Combined voltage and calcium imaging and signal calibrationMarco Canepari, Peter Saggau and Dejan ZecevicChapter 5: Use of fast-responding voltage-sensitive dyes for large-scale recording of neuronal spiking activity with single-cell resolutionWilliam N Frost, Jean Wang, Christopher J Brandon, Caroline Moore-Kochlacs, Terrence J Sejnowski and Evan S HillChapter 6: Monitoring integrated activity of individual neurons using FRET-based voltage-sensitive dyesKevin L Briggman, William B Kristan, Jesús E González, David Kleinfeld and Roger Y TsienChapter 7: Monitoring population membrane potential signals from neocortexXiaoying Huang, Weifeng Xu, Kentaroh Takagaki and Jian-young WuChapter 8: Monitoring population membrane potential signals during functional development of neuronal circuits in vertebrate embryosYoko Momose-Sato, Katsushige Sato and Kohtaro KaminoChapter 9: Imaging the dynamics of mammalian neocortical population activity in-vivoAmiram Grinvald, David Omer, Shmuel Naaman and Dahlia SharonChapter 10: Imaging the dynamics of neocortical population activity in behaving and freely moving mammalsAmiram Grinvald and Carl CH PetersenChapter 11: Monitoring membrane voltage using two-photon excitation of fluorescent voltage-sensitive dyesJonathan AN Fisher and Brian M SalzbergChapter 12: Random-access multiphoton microscopy for fast three-dimensional ImagingGaddum Duemani Reddy and Peter SaggauChapter 13: Second harmonic imaging of membrane potentialLeslie M. Loew and Aaron LewisChapter 14: Genetically encoded protein sensors of membrane potentialLei Jin, Hiroki Mutoh, Thomas Knopfel, Lawrence B Cohen, Thom Hughes, Vincent A Pieribone, Ehud Y Isacoff, Brian M Salzberg, and Bradley J Baker