Thousands of methods have been developed in the various biomedical disciplines, and those covered in this book represent the basic, essential and most widely used methods in several different disciplines.

1: Detection And Analysis Of ProteinsA. IntroductionB. Basic methods for protein analysisC. Characterization of primary, secondary, tertiaryand quaternary structures of proteins2: Detection And Analysis Of Nucleic AcidsA. IntroductionB. Basic methods for nucleic acid analysisC. DNA sequencingD. Detection and quantitation of RNA3: Recombinant DNA Techniques: Cloning And Manipulation Of DNAA. IntroductionB. Plasmid and viral vectorsC. LibrariesD. Site Directed MutagenesisE. Polymerase chain reaction (PCR)F. Screening for differentially expressed genes (DEGs)G. Promoter-protein interactionsH. Silencing gene expressionI. Forensics and DNA technology4: Antibody-Based TechniquesA. IntroductionB. Monoclonal antibodiesC. Purification and use of antibodiesD. Flow cytometry and fluorescence activated cellsorting (FACS)E. Other assays using antibodies5: Microscopy: Imaging Of Biological SpecimensA. IntroductionB. Light microscopyC. Fluorescence and laser confocal microarray microscopyD. Electron Microscopy (EM)E. Magnetic resonance imaging (MRI)6: The Derivation And Manipulation Of ExperimentalAnimals In Biomedical SciencesA. IntroductionB. Inbred strains of miceC. Congenic strains of miceD. Transgenic miceE. Targeted transgenes: 'knockout' and 'knockin' miceF. Other uses of mice in biomedical researchReferencesSome excellent methodology manualsIndex