This is the ?fth volume in the Reviews in Fluorescence series. To date, four previous volumes have been both published and well received by the scienti?c community. Several book reviews in the last few years have also favorably remarked on the series. In this ?fth volume we continue the tradition of publishing leading edge and timely articles from authors around the world. With the recent Nobel Prize in Chemistry for 2008 being awarded for the discovery and development of the Green Fluorescent Protein (GFP) to Shimomura, Chal?e, and Tsien, we have subsequently included several timely reviews on GFP in this volume. We thank the authors for their timely and exciting contributions. We hope you ?nd this volume as useful as past volumes, which promises to be just as diverse with regard to ?uorescence-based content. Finally, in closing, I would like to thank Caroleann Aitken for helping coordin- ing content with authors and Michael Weston at Springer for help in publishing this current volume. Baltimore, MD, USA Chris D. Geddes v . Contents Fluorescence Anisotropy to Study the Preferential Orientation of Fluorophores in Ordered Bi-Dimensional Systems: Rhodamine 6G/Laponite Layered Films .................. 1 F. López Arbeloa, V. Martínez, T. Arbeloa, and I. López Arbeloa Room Temperature Tryptophan Phosphorescence of Proteins in the Composition of Biological Membranes and Solutions ....... 37 Vladimir M. Mazhul¿, Alexander V. Timoshenko, Ekaterina M.

Includes supplementary material: sn.pub/extras

Fluorescence Anisotropy to Study the Preferential Orientation of Fluorophores in Ordered Bi-Dimensional Systems: Rhodamine 6G/Laponite Layered Films.- Room Temperature Tryptophan Phosphorescence of Proteins in the Composition of Biological Membranes and Solutions.- Rational Design of FRET-Based Sensor Proteins.- Fluorescence Imaging of Calcium Loading and Mitochondrial Depolarization in Cancer Cells Exposed to Heat Stress.- Energy Transfer in Silica Nanoparticles: An Essential Tool for the Amplification of the Fluorescence Signal.- Spectroscopic Characterization of Plasma - Chemically Functionalized and Fluorophore-Labeled Polymer Surfaces.- Fluorescent Labeling and Its Effect on Hybridization of Oligodeoxyribonucleotides.- New Method for Determining Histamine Rate in Halieutic Products.- Spectroscopy of DNA-Actinomycin Complexes.- Fluorescence Spectroscopy in Optoelectronics, Photomedicine, and Investigation of Biomolecular Systems.- Multicolor Imaging with Fluorescent Proteins in Mice.- Genetically Encoded Fluorescent and Bioluminescent Probes for Illuminating Cellular Signaling Pathways.- Fluorescent Protein FRET Applications.- Imaging Protein Interactions in Living Cells Using the Fluorescent Proteins.- Engineering Green Fluorescent Proteins Using an Expanded Genetic Code.- Fluorescent Proteins in Transgenic Plants.- Peptide Foldamers: From Spectroscopic Studies to Applications.- Circularly Polarized Luminescence (CPL) of Proteins and Protein Complexes.- New Dual Fluorescent Dyes Based on Modified "Excited State with Extended Conjunction" Photophysical Model.- Erratum.