This fifth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

An introduction to plant cell, tissue, and organ culture: current status and perspectives.- Use of Statistics in Plant Cell Tissue Culture.- Plant Micropropagation and Temporary Immersion Systems.- The History of Agrobacterium rhizogenes. From Pathogen to a Multitasking Platform for Biotechnology.- Use of Electron Microscopy for the Detection of Contaminant Endophytic Bacteria in vitro.- Using Mathematical Optimization Models to Improve the Efficiency of Duckweeds (Wolffia arrhiza and Lemna minor) Micropropagation.- Application of Artificial Neural Network and Genetic Algorithm Modeling for in vitro Regeneration of Seaweed Seedling Production.- Less Frequently Used Growth Regulators in Plant Tissue Culture.- Protocol for Generating Arabidopsis thaliana Cell Suspension Cultures Under Different Light Conditions.- Micropropagation of Agave angustifolia "Espadín" Used in the Production of Mezcal.- In Vitro Propagation of Threatened Agave Species Using a Two-Stage System.- In vitro Propagation of "Salsaparrilha" [Herreria salsaparilha Mart. (Asparagaceae)]: an Anti-syphilitic Medicinal Species.- Micropropagation of the Ornamental Aquatic Plant, Aponogeton ulvaceus, from Immature Tuber Explants.- Protocol for the Acclimatization of Coconut Vitro-plants Under ex-vitro Conditions.- Advances in tissue culture and transformation studies in non-model species: Passiflora spp. (Passifloraceae).- Advances in tissue culture and transformation studies in non-model species: Bixa orellana L. (Bixaceae).- Another Culture Protocols for Barley and Wheat.- Synthetic tetraploid of Oncidium crispum Lodd. (Orchidaceae).- Direct Somatic Embryogenesis in Carica papaya L. genotypes for Genetic Modification Purposes.- Scale-up of Coffea canephora Somatic Embryogenesis In Temporary Immersion System.- Large-scale Production of Specialized Metabolites in vitro Cultures.- Quantification of DNA methylation by ELISA in epigenetic studies in plant tissue culture: a theoretical-practical guide.- Proteomic Analysis Of in vitro Plant Tissues: Coffea canephora A Case Of Study.- Transcriptomic Analysis During the Induction of Somatic Embryogenesis in Coffea canephora.- Chloroplast isolation in agavaceas.- Overexpression of Genes to Enhance Abiotic Stress Tolerance in Arabidopsis.- Plant Tissue Culture and Metabolite Profiling for High-Value Natural Product Synthesis.- A Novel Method For in situ RT-PCR Based on Capsules From Centrifuge Tubes, Ideal for Transcripts Detection in Plant Tissues.- Isolation of Protoplasts from Tissues of Mexican Prickly Poppy (Argemone mexicana L.). An Alkaloid-Producing Medicinal Plant.