This third edition volume expands on the previous editions with new and updated discussions on the latest developments in endotoxin contamination, complex physicochemical properties, in vitro immunotoxicity traits, and in vitro drug release properties. Eight chapters in this book are dedicated to physicochemical characterization techniques and cover newer methods such as asymmetric-flow field-flow fractionation, single particle inductively coupled plasma mass spectrometry, and resistive pulse sensing. The next eighteen chapters explore the immunotoxicity of nanomaterials, including microbial contaminants such as endotoxin and beta-glucans, anti-PEG antibodies, autoimmunity, and immunosuppressive properties. The last two chapters talk about new pharmacology protocols, including a new technique to assess drug release and a tissue distribution assay using PEG immunohistochemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step instructions to reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Advancements in Nanoparticle Characterization.- Nanoparticle Size Distribution and Stability Assessment Using Asymmetric-Flow Field-Flow Fractionation.- Assessment of Protein Binding Using Asymmetric-Flow Field-Flow Fractionation Combined with Multi-Angle Light Scattering and Dynamic Light Scattering.- Quantitation of Active Pharmaceutical Ingredients in Polymeric Drug Products Using Elemental Analysis.- Particle Size and Concentration Measurement Using the Spectradyne nCS1 Instrument.- Measuring Size and Number Concentration of Metallic Nanoparticles Using spICP-MS.- Ion Quantitation in Liposomal Products with RP-HPLC and Charged Aerosol Detection.- A Static Headspace Gas Chromatography Method for Quantitation of Residual Solvents in Nanoformulations.- Current Considerations and Practical Solutions for Overcoming Nanoparticle Interference with LAL Assays and Minimizing Endotoxin Contamination.- Detection of Beta-Glucan Contamination inNanoparticle Formulations.- Detection of Intracellular Complement Activation by Nanoparticles in Human T Lymphocytes.- Analysis of Nanoparticles' Potential to Induce Autoimmunity.- Analysis of Nanoparticles' Effects on Drug-Induced Psoriasis.- Detection of Nanoparticle-Mediated Total Oxidative Stress in T-Cells Using CM-H2DCFDA Dye.- Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T-Cells Using MitoSOX Red Dye.- Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane Potential in T-Cells Using JC-1 Dye.- Detection of Antigen Presentation by Murine Bone Marrow-Derived Dendritic Cells After Treatment with Nanoparticles.- Antigen-Specific Stimulation of CD8+ T-Cells by Murine Bone Marrow-Derived Dendritic Cells After Treatment with Nanoparticles.- Detection of Pre-Existing Antibodies to Polyethylene Glycol and PEGylated Liposomes in Human Serum.- In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed-Type Hypersensitivity Reactions.- Analysis of Nanoparticle Adjuvant Properties.- Methods for Analysis of Nanoparticle Immunosuppressive Properties.- Evaluation of the Acute Anaphylactoid Reactogenicity of Nanoparticle-Containing Medicines and Vaccines Using the Porcine CARPA Model.- Immunophenotyping, Part I: Instrument Calibration and Reagent Qualification for immunophenotyping Analysis of Human Peripheral Blood Mononuclear Cell Cultures.- Immunophenotyping, Part II: Analysis of Nanoparticle Effects on the Composition and Activation Status of Human Peripheral Blood Mononuclear Cells.- Understanding the Role of Scavenger Receptor A1 in Nanoparticle Uptake by Murine Macrophages.- Assessment of Temperature-Dependent Drug Release of Solubilizing Nanoformulations Using the SITUA.- Evaluation of Nanomedicine Tissue Distribution and Stability by Alexa Fluor 488 and PEG Immunohistochemistry.