This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.

Includes cutting-edge techniques

Delivering Traumatic Brain Injury to Larval Zebrafish.- Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish.- Methods to Study Liver Disease Using Zebrafish Larvae.- Developmental Toxicity Assessment Using Zebrafish-Based High Throughput Screening.- Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ).- Lineage Tracing of Bone Cells in the Regenerating Fin and during Repair of Bone Lesions.- Primary Culture of Neuronal Populations for Various Downstream Applications.- Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish.- Brain Imaging and Registration in Larval Zebrafish.- Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy.- Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish.- Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows.- Generation of Conditional Knockout Zebrafish Using an Invertible Gene-Trap Cassette.- Spinal Cord Injury and Assays for Regeneration.- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase.- Section Immunostaining for Protein Expression and Cell Proliferation Studies of Regenerating Fins.- In Vivo Optogenetic Phase Transition of an Intrinsically Disordered Protein.- Colorimetric Barcoding to Track, Isolate, and Analyze Hematopoietic Stem Cell Clones.- Mutation Knock-In Methods Using Single-Stranded DNA and Gene Editing Tools in Zebrafish.- Generation of Transgenic Fish Harboring CRISPR/Cas9-Mediated Somatic Mutations via a tRNA-Based Multiplex sgRNA Expression.- Scalable CRISPR Screens in Zebrafish Using MIC-Drop.- The Goldfish Genome and Its Utility for Understanding Gene Regulation and Vertebrate Body Morphology.