This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

RNA Sequencing Analysis of Saliva exRNA.- Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry.- Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics.- Antioxidant Micronutrients and Oxidative Stress Biomarkers.- The Oral Microbiota in Health and Disease: An Overview of Molecular Findings.- The Long and Short of Genome Sequencing: Using a Hybrid Sequencing Strategy to Sequence Oral Microbial Genomes.- Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE).- Bioinformatic Approaches for Describing the Oral Microbiota.- Adhesion of Yeast and Bacteria to Oral Surfaces.- Quantitative Analysis of Periodontal Pathogens Using Real Time Polymerase Chain Reaction (PCR).- Methods to Study Antagonistic Activities Among Oral Bacteria.- Generation of Multi-Species Oral Bacteria Biofilm Models.- Markerless Genome Editing in Competent Streptococci.- A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System.- Size-Based Method for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer Patients.- Strategy for RNA-Seq Experimental Design and Data Analysis.- Characterization of the Expression and Role of Histone Acetylation and Deacetylation in Dental Pulp Cells.- Genome-Wide Analysis of Periodontal and Peri-Implant Cells and Tissues.- Differential Expression, Functional and Machine Learning Analysis of High-Throughput -Omics Data Using Open-Source Tools.- Micro-RNA Profiling in Dental Pulp Cell Cultures.- Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease.- A Cell Culture Method for the Isolation and Study of Primary Human Dental Pulp Cells.- Culturing Adipose-Derived Stem Cells Under Serum-Free Conditions.- Quantitative Real-Time Gene Profiling of Human Alveolar Osteoblasts Using a One-Step System.- Fabrication and Characterization of Decellularized Periodontal Ligament Cell Sheet Constructs.- Immunohistochemistry and Immunofluorescence.- Characterization, Quanti¿cation, and Visualization of Neutrophil Extracellular Traps.- Cell Seeding on 3D Scaffolds for Tissue Engineering and Disease Modeling Applications.- Workflow for Fabricating 3D-Printed Resorbable Personalized Porous Scaffolds for Orofacial Bone Regeneration.- Methacrylated Gelatin as an On-Demand Injectable Vehicle for Drug Delivery in Dentistry.- In Vitro Biological Testing of Dental Materials.