This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field.

Includes cutting-edge methods and protocols

Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research.- Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men.- Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection.- Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors.- Germ Cell Tumor Cell Culture Techniques .- Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops.- Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9.- Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines.- Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines.- Production and Analysis of Human Primordial Germ Cell-Like Cells.- A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors.- Targeted Methylation Analyses: From Bisulfite Treatment to Quantification.- Integrated Analysis of Germ Cell Tumors.- Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors.- A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring.- Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors.- Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors.