This third edition provides revised and expanded protocols of consolidated approaches as well as new trends in the field. Chapters guide readers through new approaches to optimize Quantum Dots' (QD) properties, to evaluate their quantum yields, important features about preparative processes and characterizations of QDs, methods related to QDs for live cell applications, and the versatility of QDs in the bioanalytical and biosensing field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Multivariate Optimization: A Powerful Tool for the Systematic Control of Quantum Dots Properties.- Absolute and Relative Methods for Fluorescence Quantum Yield Evaluation of Quantum Dots.- Preparation and Analysis of Quantum Dots - Applications of Capillary Electrophoresis.- Measuring the Hydrodynamic Radius of Colloidal Quantum Dots by Fluorescence Correlation Spectroscopy.- Encapsulating Quantum Dots in Lipid-PEG Micelles and Subsequent Copper-Free Click Chemistry Bioconjugation.- Quantitative Analysis of Single Quantum Dot Trajectories.- Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy to Prepare, Low-Cost Zwitterionic Polymer.- Dextran-Functionalized Quantum Dot-Immunoconjugates for Cellular Imaging.- Labeling Neuronal Proteins with Quantum Dots for Single Molecule Imaging.- Quantitative Chemical Delivery of Quantum Dots into the Cytosol of Cells.- Stimulus-Sensitive Theranostic Delivery Systems Based on Microcapsules Encoded with Quantum Dots and MagneticNanoparticles.- Resazurin-based Assay to Evaluate Cell Viability after Quantum Dot Interaction.- Multiplexed Detection of Cancer Serum Antigens with a Quantum Dot-Based Lab-on-Bead System.- CdS Quantum Dots Modified Photoelectrochemical Biosensor for TATA-Binding Protein Probing.- Quantum Dots-based Lateral Flow Test Strip for Glutathione Detection.- Comparative Advantages and Limitations of Quantum Dots in the Protein Array Applications.- Development of Optical Sensors Based on Quantum Dots Using Molecularly Imprinted Polymers for Determination of Prilocaine.- Detection of Tetanus Antibody Applying a Cu-Zn-In-S/ZnS Quantum Dots-based Lateral Flow Immunoassay.- Quantum Dots for Assessment of Reactive Oxygen Species Accumulation duringChemotherapy and Radiotherapy.