Methods for extraction, manipulation, and characterization of nucleic acids, peptides, and proteins have been rapidly developed. Extraction from a variety of tissues and samples have become routine and automated. Next generation sequencing (NGS) methods have been developed. This book focuses on medically-focused methods.

Preface. List of Contributors. Nucleic Acid Extraction from Diverse Samples. DNA Extraction from Mummified Tissues. Commercial DNA Extraction Kits. Automated Nucleic Acid Extraction. Forensic DNA Samples: Collection and Handling. DNA Preservation. RNA Storage. Disposable Electrochemical DNA Biosensors. Handheld Nucleic Acid Analyzer. DNA-Binding Fluorophores. Fluorescence Resonance Energy Transfer (FRET). LightUp® Probes. Invader® Assay. Laser-Capture Microdissection. Helper-Dependent Adenoviral Vectors. Retroviral Vectors. Liposomal Nonviral Delivery Vehicles. Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing. Automated DNA Sequencing. DNA Sequencing Methods. Differential Sequencing by Mass Spectrometry. Brief Guide to Conducting Biological Database Searches. Accessing Genomic Databases. Phylogenetics, Comparative Genomics, and Phylogenomics. DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences. ESTAnnotator: A Tool for High-Throughput EST Annotation. The Basics of Omics. Ribotyping. Forensic DNA Typing: Y Chromosome. Forensic Identification: An Overview on Molecular Diagnostic Technology. Denaturing Gradient Gel Electrophoresis (DGGE). Heteroduplex Analysis (HA). NEBcutter: A Program to Cleave DNA with Restriction Enzymes. Differential Display (DD) Analysis. Protein Truncation Test (PTT). Index.