Gene-based techniques have revolutionized our understanding of biology and had a direct impact on many aspects of our lives, from clinical medicine to agriculture. Route Maps takes a fresh look at this pivotal and exciting subject, within the larger context of molecular biology as a whole.

Preface About the Route Maps format The Concept Of Genes Is DevelopedGenes Are Located To ChromosomesGenes Are Composed Of DNAThe Chemical Building Blocks Of Nucelic AcidsFormation Of The DNA Double HelixPackaging Of DNA Within CellsChromatin Structure And The Functional Activity Of GenesTypes And Functions Of DNA-Protein InteractionsOrganization Of Genomes Into Multiple ChromosomesDistribution Of Nucleic Acids Within Eukaryotic CellsTypes Of RNA MoleculesThe Anatomy Of Eukaryotic ChromosomesThe Organisation Of Genes Within ChromosomesThe Molecular Anatomy Of Eukaryotic GenesChromosome Aberrations And Human DiseaseTypes Of Mutations And Their EffectsForms Of Chemically Altered DNA; DNA Repair MechanismsLinkage AnalysisPedigree Analysis And Modes Of InheritanceGenes Dictate The Nature Of ProteinsThe Nature Of The Genetic CodeTranscription: Forming Genetic MessagesPost-Transcriptional Processing Of Messenger RNATransfer And Ribosomal RNA Processing/ModificationMechanisms Regulating Gene ExpressionTranscriptional Regulatory SequencesOperons And Prokaryotic Control Of Gene ExpressionTranscription Factors And Gene ExpressionIn Vivo Translation: Decoding Genetic MessagesSequences Involved In Cellular Protein TargetingEukaryotic Cell Division: Mitosis And MeiosisMolecular Mechanisms Of Cell Cycle ControlGenetic Recombination MechanismsGene Transfer During Bacterial ReproductionTransposable Genetic Elements: TransposonsIn Vivo DNA ReplicationGenetic Control Of DevelopmentThe Natural Biology Of BacteriophagesBacteriophage GeneticsRecombinant DNA TechnologyEnzymes Commonly Used In Molecular Biology MethodsRestriction EndonucleasesRestriction Fragment Length PolymorphismsIsolation Of Nucleic Acids From Cells And TissuesVisualising Mucleic AcidsElectrophoresis Of Nucleic AcidsIn Vitro HybridisationTypes Of Hybridisation Assay FormatsSouthern Blotting; In Situ HybridisationMeasuring Transcriptional Activity Via Messenger RNAConverting Messenger RNA Into Complementary DNA (Cdna)Methods For Determining DNA Nucleotide SequencesThe Polymerase Chain ReactionAlternatives To PCR-Based In Vitro DNA/RNA AmplificationIn Vitro Translation MethodsTypes And Methods Of Gene Probe GenerationChemical Synthesis Of OligonucleotidesTypes And Applications Of Nucleotide AnaloguesMethods For Labelling Gene ProbesFundamental Principles Of CloningThe Nature Of Cloning VectorsInserting Foreign DNA Into VectorsThe Development Of Bacteriophage VectorsPlasmids: Development As Clonign VectorsYeast-Derived Plasmid VectorsPhagemids: Hybrid Phage And Plasmid VectorsVectors For Use In Plant And Animal CellsDelivering DNA Into Cells; Principal Genomic And Cdna Cloning StrategiesStrategies For Identifying Desirable Recombinant ClonesGene Mapping TechniquesDetecting DNA-Protein Interaction SitesDetecting Promoter And Enhancer SequencesMethods For Identifying Protein Encoding SequencesGenetic FingerprintingAnalysing Ancient DnasIn Vitro Mutagenesis MethodsGenetically Modified Micro-OrganismsGenetically Engineered PlantsGenetically Engineered AnimalsMolecular Techniques In Prenatal DiagnosisThe Genetics Of Cystic FibrosisThe Dystrophin Gene And Muscular DystrophiesIdentifying The Gene For Huntingdon's DiseaseLipoprotein Genes And Heart DiseaseThe Detection Of Microbial InfectionsMolecular Biology Of Human Immunodeficiency Virus And AIDSEngineering Microbial BioluminescenceRecombinant DNA Techniques In Vaccine DevelopmentReceptors And Cellular Signalling MechanismsOncogenes And The Molecular Basis Of CancerMolecular Diagnosis And Therapy Of CancersDrug Development Using Recombinant DNA ApproachesProtein EngineeringImmunoglobulin GeneticsGenetic Engineering Of Recombinant AntibodiesCurrent Approaches To Gene TherapyThe Human Genome Mapping ProjectBibliography Index