Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.

Contributors.Preface.SECTION 1 Agrobacterium-MediatedTransformation.Chapter 1 Host Factors Involved in Genetic Transformation ofPlant Cells byAgrobacterium (BENO^IT LACROIX, ADI ZALTSMAN,AND VITALY CITOVSKY).Chapter 2 Genomics of Agrobacterium-PlantInteraction: An Approach to Refine the Plant TransformationTechnology (AJITH ANAND, ZARIR E. VAGHCHHIPAWALA, ANDKIRANKUMAR S. MYSORE).SECTION 2 Other Transformation Technologies.Chapter 3 Particle Bombardment: An Established Weapon in theArsenal of Plant Biotechnologists (THEODORE M. KLEIN).Chapter 4 A Novel Gene Delivery System in Plants with CalciumAlginate Micro-Beads (NAOKI WADA, SHIN'ICHIRO KAJIYAMA,NARUEMON KHEMKLADNGOEN, AND KIICHI FUKUI).Chapter 5 Pollen Transformation Technologies (TATIANARESCH AND ALISHER TOURAEV).Chapter 6 Intragenic Vectors and Marker-Free Transformation:Tools for a Greener Biotechnology (CAIUS M. ROMMENS, ANTHONYCONNER, HUA YAN, AND ZAC HANLEY).Chapter 7 Visualizing Transgene Expression (JOHN J.FINER).SECTION 3 Vectors, Promoters, and Other Tools for PlantTransformation.Chapter 8 Current State and Perspective of Binary Vectors andSuperbinary Vectors (TOSHIYUKI KOMORI AND TOSHIHIKOKOMARI).Chapter 9 Novel Dual Binary Vectors (pCLEAN) for PlantTransformation (PHILIPPE VAIN).Chapter 10 pORE Modular Vectors for Plant Transformation(DWAYNE D. HEGEDUS).Chapter 11 pANIC: A Versatile Set of Gateway-CompatibleVectors for Gene Overexpression and RNAi-Mediated Down-Regulationin Monocots (DAVID G.J. MANN, PETER R. LAFAYETTE, LAURA L.ABERCROMBIE, WAYNE A. PARROTT, AND C. NEAL STEWART, JR.).SECTION 4 Transgene Integration, Stability, Methylation,Silencing.Chapter 12 Understanding and Avoiding Transgene Silencing(MICHAEL W. GRAHAM, STEPHEN R. MUDGE, PETER R. STERNES, ANDROBERT G. BIRCH).Chapter 13 Site-Specific Recombination for Precise and"Clean" Transgene Integration in Plant Genome(VIBHA SRIVASTAVA AND DAVID W. OW).SECTION 5 Selection Systems, Marker-FreeTransformation.Chapter 14 Selectable Marker Genes: Types and Interactions(BRIAN MIKI, ASHRAF ABDEEN, YUZUKI MANABE, SOUAD EL OUAKFAOUI,DOUGLAS A. JOHNSON, LORETA GUDYNAITE-SAVITCH, MING HU, AND PHILMACDONALD).Chapter 15 Transformation Methods for Obtaining Marker-FreeGenetically Modified Plants (JAN G. SCHAART, FRANS A. KRENS,ANNE-MARIE A. WOLTERS, AND RICHARD G.F. VISSER).Chapter 16 Intellectual Property Aspects of PlantTransformation (JIM M. DUNWELL).Index.Color Plate between pages 82 and 83.