This book provides a detailed description of various multidimensional chromatographic separation techniques. The editor first provides an introduction to the area and then dives right into the various complex separation techniques. While still not used routinely comprehensive chromatography techniques will help acquaint the readers with the fundamentals and possible benefits of multi-dimensional separations coupled with mass spectrometry.The topics include a wide range of material that will appease all interested in either entering the field of multidimensional chromatography and those looking to gain a better understanding of the topic.

Contributors ixPreface xi1 Introduction 1Luigi Mondello, Peter Q. Tranchida, and Keith D. Bartle1.1 Two-Dimensional Chromatography-Mass Spectrometry: A 50-Year-Old Combination, 21.2 Shortcomings of One-Dimensional Chromatography, 31.3 Benefits of Two-Dimensional Chromatography, 61.4 Book Content, 71.5 Final Considerations, 112 Multidimensional Gas Chromatography: Theoretical Considerations 13Leonid M. Blumberg2.1 Symbols, 152.2 One-Dimensional GC, 172.3 Comprehensive GC × GC, 313 Multidimensional Liquid Chromatography: Theoretical Considerations 65Pavel Jandera3.1 Two-Dimensional LC Techniques, 663.2 Peak Capacity in HPLC: One- and Multidimensional Separations, 693.3 Orthogonality in Two-Dimensional LC-LC Systems, 733.4 Sample Dimensionality and Structural Correlations, 753.5 Separation Selectivity and Selection of Phase Systems in Two-Dimensional LC-LC, 793.6 Programmed Elution in Two-Dimensional HPLC, 813.7 Fraction Transfer Modulation in Comprehensive LC × LC: Additional Band Broadening, 873.8 Future Perspectives, 894 History, Evolution, and Optimization Aspects of Comprehensive Two-Dimensional Gas Chromatography 93Ahmed Mostafa, Tadeusz G¿orecki, Peter Q. Tranchida, and Luigi Mondello4.1 Fundamentals of GC × GC, 944.2 Modulation, 964.3 GC × GC Data Interpretation, 974.4 GC × GC Instrumentation, 1004.5 Thermal Modulators, 1014.6 Comprehensive Two-Dimensional GC Method Optimization, 1154.7 Final Remarks, 1405 Flow-Modulated Comprehensive Two-Dimensional Gas Chromatography 145John V. Seeley5.1 Timing Requirements of GC × GC Modulators, 1455.2 Criteria for Evaluating Modulators, 1505.3 Forms of Modulation, 1515.4 Single-Stage Flow Modulation, 1525.5 Two-Stage Flow Modulation, 1575.6 Summary of Flow Modulators, 1675.7 Brief Comparison to Thermal Modulation, 1695.8 Concluding Remarks, 1696 Comprehensive Two-Dimensional Gas Chromatography Combined with Mass Spectrometry 171Peter Q. Tranchida, Luigi Mondello, Samuel D. H. Poynter, and Robert A. Shellie6.1 Instrument Requirements for GC × GC-MS, 1736.2 Data Processing of GC × GC-TOF MS Results, 1806.3 Method Translation in GC × GC-MS, 1816.4 GC × MS, 1816.5 Conventional and Alternative Modulation Techniques for GC × GC-MS, 1846.6 GC × GC-MS Applications, 1876.7 Concluding Remarks, 2327 Detector Technologies and Applications in Comprehensive Two-Dimensional Gas Chromatography 243Philip J. Marriott7.1 Detection in GC × GC, 2447.2 Comments on GC × GC with Mass Spectrometry, 2497.3 Flame Ionization Detection in GC × GC, 2507.4 Electron Capture Detection in GC × GC, 2547.5 Sulfur Chemiluminescence Detection in GC × GC, 2607.6 Nitrogen Chemiluminescence Detection in GC × GC, 2637.7 Atomic Emission Detection in GC × GC, 2657.8 Thermionic Detection in GC × GC, 2677.9 Flame Photometric Detection in GC × GC, 2697.10 Case Study of GC × GC with Selective Detection, 2727.11 Dual Detection with GC × GC, 2747.12 Conclusions, 2758 History, Evolution, and Optimization Aspects of Comprehensive Two-Dimensional Liquid Chromatography 281Isabelle Fran¿cois, Koen Sandra, and Pat Sandra8.1 Method Development and Instrumentation, 2828.2 Technical Problems in Comprehensive Liquid Chromatography, 3098.3 Detection, 3148.4 Data Representation, 3158.5 Instrumentation, 3168.6 Milestones in Comprehensive Liquid Chromatography, 3178.7 Applications, 3208.8 Beyond Two-Dimensional Chromatography, 3208.9 Comparison of LC × LC and Off-Line 2D LC, 3218.10 Conclusions, 3259 Comprehensive Two-Dimensional Liquid Chromatography Combined with Mass Spectrometry 331Paola Dugo, Luigi Mondello, Francesco Cacciola, and Paola Donato9.1 HPLC-MS, 3329.2 LC × LC-MS Instrumentation and Method Development, 3349.3 LC × LC-MS Applications, 33610 Comprehensive Two-Dimensional Liquid Chromatography Applications 391Paola Dugo, Luigi Mondello, Francesco Cacciola, and Paola Donato10.1 Comprehensive 2D LC Separation of Synthetic and Natural Polymers, 39210.2 Comprehensive 2D LC Separation of Natural Products and Antioxidants, 39910.3 Comprehensive 2D LC Separation of Pharmaceutical and Environmental Compounds, 41210.4 Comprehensive 2D LC Separation of Proteins and Peptides, 41711 Other Comprehensive Chromatography Methods 429Isabelle Fran¿cois, Pat Sandra, Danilo Sciarrone, and Luigi Mondello11.1 Online Two-Dimensional Liquid Chromatography-Gas Chromatography, 43011.2 Online Two-Dimensional Supercritical Fluid Chromatography-Gas Chromatography, 44011.3 Online Two-Dimensional Supercritical Fluid Chromatography-Supercritical Fluid Chromatography, 44011.4 Online Two-Dimensional Supercritical Fluid Chromatography-Liquid Chromatography, 44212 Comprehensive Chromatography Data Interpretation Technologies 449Elizabeth M. Humston and Robert E. Synovec12.1 Higher-Order Data Structure, 45012.2 Modifications of First-Order Data-Handling Approaches, 45312.3 Visualization, 45612.4 Mass Spectral Detection, 46112.5 Chemometrics, 46312.6 Summary of Data Interpretation Technologies, 472Index 477