This book is designed to be a central text for young graduate students interested in mass spectrometry as it relates to study of protein structure and function as well as proteomics. It is a definite must have work for: - libraries at academic institutions with Master and Graduate programs in Biochemistry, Molecular Biology, Structural Biology and Proteomics;- individual laboratories with interests covering these areas; and - libraries and individual laboratories in the pharmaceutical and biotechnology industries. . serves as an essential reference to those working in the field. incorporates the contributions of prominent experts . features comprehensive coverage and a logical structure

Foreword Chapter 1. An Introduction to the Basic Principles and Concepts of Mass Spectrometry Kym Faull, Alek N. Dooley, Frederic Halgand, Lorelei D. Shoemaker, Andrew J. Norris, Christopher M. Ryan, Arthur Laganowsky, Jodie V. Johnson and Jonathan E. Katz Chapter 2. Charaterization of protein conformation and dynamics with ESI MSIgor Kaltashov and Wendell P. GriffithChapter 3. An Introduction to Ion Mobility and Mass Spectrometry of Non-Covalent Protein Complexes Summer Bernstein, and Michael BowersChapter 4. Study of protein structure using hydrogen-deuterium exchangeJennifer L. Mitchell and John R. EngenChapter 5. Stopped flow analysis with MSLars Konermann, Jingxi Pan, and Derek J. WilsonChapter 6. SUPREX; Stability of Unpurified Proteins by Rates of H/D ExchangeMichael C. Fitzgerald, Liangjie Tang, and Erin D. HopperChapter 7. Microsecond time-scale hydroxyl radical profiling of solvent-accessible protein residuesDavid Hambly and Michael GrossChapter 8. Intact Protein Mass Measurements and Top-Down Mass Spectrometry: Application to Integral Membrane Proteins. Julian P. WhiteleggeChapter 9. Analysis of dynamic membrane protein structure-function relationships using chemical modification techniques and mass spectrometryAdam B. Weinglass,Chapter 10. Shotgun approaches to membrane protein structure and topologyAnna E. Speers and Christine WuChapter 11. Covalent trapping of protein interactions in complex systemsRasanjala Weerasekera, Tujin Shi and Gerold Schmitt-UlmsChapter 12. PhosphoproteomicsMartin R. Larsen and Phillip J. RobinsonChapter 13. Detection of tyrosine phosphorylation sites by mass spectrometryGuoan Zhang, Chongfeng Xu and Thomas A. NeubertChapter 14. Histidine PhosphorylationXin-Lin Zu, Paul G. Besant and Paul V. AttwoodChapter 15. O-glcnac modification of proteinsRobert J. Chalkley, Lance Wells and Keith VossellerChapter 16. Analysis of Deamidation in ProteinsJason J. Cournoyer and Peter B. O'ConnorChapter 17. Quantitative ProteomicsSilke Oeljeklaus, Jon Barbour and Bettina WarscheidChapter 18. Multiplexed Quantitative Proteomics using Mass SpectrometryPhilip L. Ross, Xunming Chen, Esteban Toro, Leticia Britos, Lucy Shapiro, Darryl PappinChapter 19. Large-scale subcellular localization of proteins by protein correlation profiling.Leonard J. FosterChapter 20. Accuracy of Quantitation from Full and Partial Metabolic Labeling ExperimentsEdward L. Huttlin, Adrian D. Hegeman and Michael R. Sussman, Ph.D.