The authors offer novel insights into bioreactor-based culture systems specific for tissue engineering, including sophisticated and cost-effective manufacturing strategies geared to overcome technological shortcomings that currently preclude advances towards product commercialization.

Expansion of human pluripotent stem cells in microcarrier-based culture systems. Large-scale culture of 3D aggregates of pluripotent stem cells. Cardiac differentiation of human pluripotent stem cells in scalable suspension culture. Integrated processes for expansion and differentiation of human pluripotent stem cells into neural progenitor cells. Bioreactor protocols for human neural precursor cell expansion. Expansion of human cardiac progenitor cells in using microcarriers. Differentiation of embryonic stem cells towards red blood cell production. Expansion of hematopoietic progenitor cells in a hollow fiber bioreactor system. Engineering the bone marrow niche for platelet generation ex vivo and modeling of megakaryopoiesis. Packed Bed Bioreactor for the Isolation and Expansion of Human Mesenchymal Stem/Stromal Cells. Expansion of human mesenchymal stem/stromal cells in microcarrier-based culture systems. Scaffold-based perfusion bioreactors for Cell Therapies and Tissue Engineering. Vascular Tissue Engineering. Bioreactor design for bone and cartilage tissue engineering. Engineering cardiac tissue in bioreactors. Organ-level tissue engineering using bioreactor systems and stem cells. Kidney Tissue Engineering. Liver Tissue Engineering. Bioprocess monitoring in tissue engineering perfusion bioreactors. Bioprocessing of human stem cells for therapeutic use through single-use bioreactors.