All enzymes are remarkable since they have the ability to increase the rate of a chemical reaction, often by more than a billion-fold. Allosteric enzymes are even more amazing because the have the additional ability to change their rate in response to cellular activators or inhibitors. This enables them to control the pathway in which they are the regulatory enzyme. Since the effector molecules represent the current status of the cell for a given metabolic pathway, this results in very responsive and balanced metabolic states, and makes it possible for cells and organisms to be appropriately dynamic, and responsive, in a changing environment. This book provides a logical introduction to the limits for enzyme function as dictated by the factors that are limits for life. This book presents a complete description of all the mechanisms used for changing enzyme acticity. Eight enzymes are used as model systems after extensive study of their mechanisms. Wherever possible, the human form of the enzyme is used to illustrate the regulatory features.

"All enzymes are remarkable since they have the ability to increase the rate of a chemical reaction, often by more than a billion-fold. Allosteric enzymes are even more amazing because the have the additional ability to change their rate in response to cellular activators or inhibitors. This enables them to control the pathway in which they are the regulatory enzyme. Since the effector molecules represent the current status of the cell for a given metabolic pathway, this results in very responsive and balanced metabolic states, and makes it possible for cells and organisms to be appropriately dynamic, and responsive, in a changing environment. This book provides a logical introduction to the limits for enzyme function as dictated by the factors that are limits for life. This book presents a complete description of all the mechanisms used for changing enzyme acticity. Eight enzymes are used as model systems after extensive study of their mechanisms. Wherever possible, the human form of the enzyme is used to illustrate the regulatory features. TOC:General Introduction; Regulation of Metabolism.- Overview of Enzyme Structure with emphasis on domains, and position of catalytic sites at domain interface or subunit interface.- Description of Enzyme Assays, with emphasis on choosing methods for time.- Enzyme Kinetics.- Types of Cooperativity.- Hemoglobin as a Model System of a classic homo- oligomer.- Aspartate transcarbamylase, E coli, as a classic hetero-oligomer. Example of separate sites for positive and negative regulatory effectors.- Phosphofructokinase.- Glycogen Phosphorylase.- Hexokinase, yeast and rat.- Amidophosphoribosyltransferase.- Ribonucleotide reductase.- Protein Engineering and Future Directions.- Discussion.- References.- Index."

Overview Of Enzymes.- To Enzymes.- The Limits For Life Define The Limits For Enzymes.- Enzyme Kinetics.- Properties And Evolution Of Allosteric Enzymes.- Kinetics Of Allosteric Enzymes.- K-Type Enzymes.- Hemoglobin.- Glycogen Phosphorylase.- Phosphofructokinase.- Ribonucleotide Reductase.- Hexokinase.- V-Type Enzymes.- To V-Type Enzymes.- G Proteins.- Protein Kinases.