The first two editions of this title had a tremendous impact in neuroscience. Between the Second edition in 1989 and today, there has been an explosion of information in the field, including advances in molecular techniques, such as genomics and proteomics, which have become increasing important in neuroscience. A renaissance in fluorescence has occurred, driven by the development of new probes, new microscopes, live imagers, and computer processing. The introduction of new markers has enormously stimulated the field, moving it from tissue culture to neurophysiology to functional MRI techniques.

The first two editions of this title had a tremendous impact in neuroscience. Between the previous edition in 1989 and today, there has been an explosion of information in the field. These include advances in molecular techniques, such as genomics and proteomics, which have become increasing important in neuroscience. A renaissance in fluorescence has occurred, driven by the development of new probes, new microscopes, live imagers, and computer processing. The introduction of new markers has enormously stimulated the field, moving it from tissue culture to neurophysiology to functional MRI techniques.

Short Retrospection.- Preembedding Immunoelectron Microscopy: Applications for Studies of the Nervous System.- Postembedding Immunogold Cytochemistry of Membrane Molecules and Amino Acid Transmitters in the Central Nervous System.- Cell and Tissue Microdissection in Combination with Genomic and Proteomic Applications.- Molecules and Membrane Activity: Single-Cell RT-PCR and Patch-Clamp Recording from Central Neurons.- Merging Structure and Function: Combination of In Vivo Extracellular and Intracellular Electrophysiological Recordings with Neuroanatomical Techniques.- Juxtacellular Labeling of Individual Neurons In Vivo: From Electrophysiology to Synaptology.- Nonradioactive In Situ Hybridization in Combination with Tract-Tracing.- Viral Tracers for the Analysis of Neural Circuits.- Dextran Amines: Versatile Tools for Anterograde and Retrograde Studies of Nervous System Connectivity.- Multiple Neuroanatomical Tract-Tracing: Approaches for Multiple Tract-Tracing.- Tract-Tracing in Developing Systems and in Postmortem Human Material Using Carbocyanine Dyes.- Combined Fluorescence Methods to Determine Synapses in the Light Microscope: Multilabel Confocal Laser Scanning Microscopy.- Advances in Understanding Cortical Function Through Combined Voltage-Sensitive Dye Imaging, Whole-Cell Recordings, and Analysis of Cellular Morphology.- From Dendrites to Networks: Optically Probing the Living Brain Slice and Using Principal Component Analysis to Characterize Neuronal Morphology.- Stereology of Neural Connections: An Overview.- Three-Dimensional Computerized Reconstruction from Serial Sections: Cell Populations, Regions, and Whole Brain.- Atlases of the Human Brain: Tools for Functional Neuroimaging.- Neuron and Network Modeling.- Functional Connectivity of the Brain: Reconstruction from Static and Dynamic Data.