Thousands of methods have been developed in the various biomedical disciplines, and those covered in this book represent the basic, essential and most widely used methods in several different disciplines.

Thousands of methods have been developed in the various biomedical disciplines and those covered in this book represent the basic, essential and most widely used methods in several different disciplines.

1: Detection And Analysis Of ProteinsA. IntroductionB. Basic methods for protein analysisC. Characterization of primary, secondary, tertiaryand quaternary structures of proteins2: Detection And Analysis Of Nucleic AcidsA. IntroductionB. Basic methods for nucleic acid analysisC. DNA sequencingD. Detection and quantitation of RNA3: Recombinant DNA Techniques: Cloning And Manipulation Of DNAA. IntroductionB. Plasmid and viral vectorsC. LibrariesD. Site Directed MutagenesisE. Polymerase chain reaction (PCR)F. Screening for differentially expressed genes (DEGs)G. Promoter-protein interactionsH. Silencing gene expressionI. Forensics and DNA technology4: Antibody-Based TechniquesA. IntroductionB. Monoclonal antibodiesC. Purification and use of antibodiesD. Flow cytometry and fluorescence activated cellsorting (FACS)E. Other assays using antibodies5: Microscopy: Imaging Of Biological SpecimensA. IntroductionB. Light microscopyC. Fluorescence and laser confocal microarray microscopyD. Electron Microscopy (EM)E. Magnetic resonance imaging (MRI)6: The Derivation And Manipulation Of ExperimentalAnimals In Biomedical SciencesA. IntroductionB. Inbred strains of miceC. Congenic strains of miceD. Transgenic miceE. Targeted transgenes: 'knockout' and 'knockin' miceF. Other uses of mice in biomedical researchReferencesSome excellent methodology manualsIndex