Beschreibung:
This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
Preface, Contributors, Introduction, 1. Release of Protein from Biological Host, 2. Chromatography: The High-Resolution Technique for Protein Separation, 3. Selectivity in Affinity Chromatography, 4. Genetic Approaches to Facilitate Protein Purification, 5. Solid-Liquid Separation, 6. Membranes for Protein Isolation and Purification, 7. Precipitation of Proteins: Nonspecific and Specific, 8. Protein Crystallization for Large-Scale Bioseparation, 9. Extraction for Rapid Protein Isolation, 10. Adsorption as an Initial Step for the Capture of Proteins, 11. Fast Chromatography of Proteins, 12. Novel Approaches to the Chromatography of Proteins, 13. Scale-up and Commercial Production of Recombinant Proteins, 14. Process Monitoring During Protein Purification, 15. Protein Formulation, 16. Regulatory Requirements, Cleaning, and Sanitization Issues in Protein Purification, Index