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Advanced Methods in Molecular Biology and Biotechnology
ISBN-13:
9780128244500
Veröffentl:
2020
Seiten:
218
Autor:
Khalid Z. Masoodi
eBook Typ:
EPUB
eBook Format:
EPUB
Kopierschutz:
2 - DRM Adobe
Sprache:
Englisch
Beschreibung:
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.
1. Introduction to molecular biology techniques 2. Agarose gel electrophoresis 3. SDS-polyacrylamide gel electrophoresis 4. Immunoelectrophoresis 5. DNA plasmid isolation using Alkaline Lysis Method from E. coli DH5a cells 6. Restriction digestion of Plasmid DNA 7. Genomic DNA extraction from plant leaves using CTAB method 8. Genomic DNA extraction from silkworm using modified phenol: chloroform method (Suzuki et al., 1972) 9. Genomic DNA extraction from Fungal Mycelium using modified Cenis, 1992 method 10. Bacterial transformation protocol using CaCl2
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