Advanced Methods in Molecular Biology and Biotechnology
A Practical Lab Manual
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ISBN-13:
9780128244494
Veröffentl:
2020
Erscheinungsdatum:
30.10.2020
Seiten:
218
Autor:
Khalid Z Masoodi
Gewicht:
460 g
Sprache:
Englisch
Beschreibung:
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.
1. Introduction to molecular biology techniques2. Agarose gel electrophoresis3. SDS-polyacrylamide gel electrophoresis4. Immunoelectrophoresis5. DNA plasmid isolation using Alkaline Lysis Method from E. coli DH5a cells6. Restriction digestion of Plasmid DNA7. Genomic DNA extraction from plant leaves using CTAB method8. Genomic DNA extraction from silkworm using modified phenol: chloroform method (Suzuki et al., 1972)9. Genomic DNA extraction from Fungal Mycelium using modified Cenis, 1992 method10. Bacterial transformation protocol using CaCl2
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