Enzymes as Sensors, Volume 589, the latest release in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics that presents a micro-level delivery of specific content related to the study of enzymes in sensors. New to this edition are highly specialized chapters on integrated strategies for gaining a systems level view of dynamic signaling networks, sensitive protein detection and quantification in paper-based microfluidics for point-of-care, and microneedle enzyme sensor arrays for continuous in vivo monitoring.

1. Recent Advances in Development of Genetically Encoded Fluorescent Sensors 2. Engineering Rugged Field Assays to Detect Hazardous Chemicals Using Spore-Based Bacterial Biosensors 3. Engineering BRET-Sensor Proteins 4. Enzymes as Sensors 5. Integrated Strategies to Gain a Systems-Level View of Dynamic Signaling Networks 6. Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor 7. Novel Fluorescence-Based Biosensors Incorporating Unnatural Amino Acids 8. Folding- and Dynamics-Based Electrochemical DNA Sensors 9. Construction of Protein-Based Biosensors Using Ligand-Directed Chemistry for Detecting Analyte Binding 10. Measuring and Imaging Metal Ions With Fluorescence-Based Biosensors: Speciation, Selectivity, Kinetics, and Other Issues 11. Bioapplications of Electrochemical Sensors and Biosensors 12. A Highly Sensitive Biosensor for ATP Using a Chimeric Firefly Luciferase 13. Highly Modular Bioluminescent Sensors for Small Molecules and Proteins 14. Sensitive Protein Detection and Quantification in Paper-Based Microfluidics for the Point of Care 15. Microneedle Enzyme Sensor Arrays for Continuous In Vivo Monitoring 16. Visualization of the Genomic Loci That Are Bound by Specific Multiprotein Complexes by Bimolecular Fluorescence Complementation Analysis on Drosophila Polytene Chromosomes