Synthetic Biology and Metabolic Engineering in Plants and Microbes: Part A, the new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.

1. Directing Biosynthesis: Practical Supply of Natural and Unnatural CyanobactinsDebosmita Sardar, Ma Diarey Tianero and Eric W. Schmidt2. Synthetic Biology Approaches to New BisindolesLona M. Alkhalaf, Yi-Ling Du and Katherine S. Ryan3. Enzymatic [4+2] Cycloadditions in the Biosynthesis of Spirotetramates and SpirotetronatesBo Pang, Guannan Zhong, Zhijun Tang and Wen Liu4. Application and Modification of Flavin-Dependent HalogenasesKarl-Heinz van Pée, Daniela Milbredt, Eugenio Patallo, Veit Weichold and Maria Gajewi5. Engineering Flavin-Dependent HalogenasesJames T. Payne, Mary C. Andorfer and Jared C. Lewis6. Heterologous Expression of Fungal Secondary Metabolite Pathways in the Aspergillus Nidulans Host SystemJohannes W. A. van Dijk and Clay C. C. Wang7. Plug-and-Play Benzylisoquinoline Alkaloid Biosynthetic Gene Discovery in Engineered YeastJeremy S. Morris, Mehran Dastmalchi, Jing Li, Limei Chang, Xue Chen, Jillian M. Hagel and  Peter J. Facchini8. Optimizing Metabolic Pathways for the Improved Production of Natural ProductsJ. Andrew Jones and Mattheos A. G. Koffas9. Reconstituting Plant Secondary Metabolism in Saccharomyces Cerevisiae for Production of High-Value Benzylisoquinoline AlkaloidsMichael E. Pyne, Lauren Narcross, Elena Fossati, Leanne Bourgeois, Euan Burton, Nicholas D. Gold and Vincent J.J. Martin10. Engineering Microbes to Synthesize Plant IsoprenoidsKang Zhou, Steven Edgar and Gregory Stephanopoulos11. Natural Product Biosynthesis in Escherichia Coli: Mentha MonoterpenoidsHelen S. Toogood, Shirley Tait, Adrian Jervis, Aisling Ní Cheallaigh, Luke Humphreys, Eriko Takano, John M. Gardiner and Nigel S. Scrutton12. High-Efficiency Genome Editing of Streptomyces Species by an Engineered Crispr/Cas SystemYajie Wang, Ryan E. Cobb and Huimin Zhao13. Rapid optimization of engineered metabolic pathways with Serine Integrase Recombinational Assembly (SIRA)Christine Merrick, Caroline Wardrope, Jane Paget, Sean Colloms and Susan Rosser14. Rewiring Riboswitches to Create New Genetic Circuits in BacteriaChristopher J. Robinson, Daniel Medina-Stacey, Ming-Cheng Wu, Helen A. Vincent and Jason Micklefield