Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments.

Part I:  General Methods to Study IF Proteins1. How to Study Intermediate Filaments in Atomic DetailAnastasia A. Chernyatina, John F. Hess, Dmytro Guzenko, John C. Voss and Sergei V. Strelkov2. Mechanical Properties of Intermediate Filament ProteinsElisabeth E. Charrier and Paul A. Janmey3. Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and TissuesNicole Schwarz, Marcin Moch, Reinhard Windoffer and Rudolf E. Leube4. Phospho-Specific Antibody Probes of Intermediate Filament (IF) ProteinsHidemasa Goto, Hiroki Tanaka, Kousuke Kasahara  and Masaki Inagaki5. Assays for Post-Translational Modifications of Intermediate Filament ProteinsNatasha T. Snider and M. Bishr Omary6. Immunofluorescence and Immunohistochemical Detection of KeratinsCornelia Stumptner, Margit Gogg-Kamerer, Christian Viertler, Helmut Denk and Kurt Zatloukal7. High Throughput Screening for Drugs that Modulate Intermediate Filament ProteinsJingyuan Sun , Vincent E. Groppi , Honglian Gui, Lu Chen, Qing Xie, Li Liu and M. Bishr Omary8. The Use of Withaferin A to Study Intermediate FilamentsRoyce Mohan and Paola BargagnaMohan9. Assays to Study Consequences of Cytoplasmic Intermediate Filament Mutations: the Case of Epidermal KeratinsTong San Tan, Yi Zhen Ng, Cedric Badowski, Tram Dang, John E A Common, Lukas Lacina, Ildikó Szeverényi and E Birgitte Lane10. Using Data Mining and Computational Approaches to Study Intermediate     Filament Structure and FunctionDavid A.D. ParryPart II: Mammalian IF Proteins11. Isolation and Analysis of Keratins and Keratin Associated Proteins from Hair and WoolSantanu Deb-Choudhury, Jeffrey E. Plowman and Duane P. Harland12. Skin KeratinsFengrong Wang, Abigail Zieman and Pierre A. Coulombe13. Simple Epithelial KeratinsPavel Strnad, Nurdan Guldiken, Terhi O. Helenius, Julia O. Misiorek, Joel H. Nyström, Iris A.K. Lähdeniemi, Jonas S.G. Silvander, Deniz Kuscuoglu and Diana M. Toivola14. Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments Karen M. Ridge, Dale Shumaker, Amélie Robert, Caroline Hookway, Vladimir I. Gelfand, Paul A. Janmey, Jason Lowery, Ming Guo, David A. Weitz, Edward Kuczmarski, and Robert D. Goldman15. Strategies to Study Desmin  in Cardiac Muscle and Culture Systems Antigoni Diokmetzidou, Mary Tsikitis, Sofia Nikouli, Ismini Kloukina, Elsa Tsoupri, Stamatis Papathanasiou, Stelios Psarras, Manolis Mavroidis and Yassemi Capetanaki16. Genetic Manipulation of Neurofilament Protein PhosphorylationMaria R. Jones, Eric Villalón and Michael L. Garcia17. a-Internexin and Peripherin: Expression, Assembly, Functions and Roles in DiseaseJian Zhao and Ronald K. H. Liem18. Studying Nestin and its Inter-Relationship with Cdk5Julia Lindqvist, Num Wistbacka and John E. Eriksson19. Synemin: Molecular Features and the Use of Proximity Ligation Assay to Study its InteractionsMadhumita Paul and Omar Skalli20. Targeting Mitogen-Activated Protein Kinase Signaling in Mouse Models of Cardiomyopathy Caused By Lamin A/C Gene MutationsAntoine Muchir and Howard J Worman21. In Vivo, Ex Vivo and in Vitro Approaches to Study Intermediate Filaments in the Eye LensM. Jarrin, L. Young, W. Wu, J.M. Girkin and R.A. QuinlanPart III:  Non-Mammalian IF Protein Systems22. Compartment Specific Phosphorylation of Squid NeurofilamentsPhilip Grant and Harish C. Pant23. Using Xenopus Embryos to Study Transcriptional and Post-Transcriptional Gene Regulatory Mechanisms of Intermediate FilamentsChen Wang and Ben G. Szaro24. Intermediate Filaments in Caenorhabditis ElegansNoam Zuela and Yosef Gruenbaum25. Mechanical Probing of the Intermediate Filament-Rich C. Elegans IntestineOliver Jahnel, Bernd Hoffmann, Rudolf Merkel, Olaf Bossinger and  Rudolf E. Leube26. Using Drosophila for Studies of Intermediate FilamentsJens Bohnekamp, Diane E. Cryderman, Dylan A. Thiemann, Thomas M. Magin and Lori L. Wallrath