The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.

[Contents in a condensed version]Basic MethodologyAmplification of genomic DNA targetsOptimization of PCR reaction and design of primersNonradioactive detection of PCR productsmRNA quantitation by PCRCloning PCR productsContamination and product carry-overPCR from paraffin sectionsAmplifying ancient DNAResearch ApplicationsDirect DNA sequencing of PCR productsDNA footprintingDetection of rare mRNAsSite-directed mutagenesisScreening DNA librariesAmplification of flanking DNA sequencesGenetics and EvoltionTissue typing and disease susceptibilityHaplotype analysis of single cellsPopulation biologyMultiplex DNA amplificationDiagnostics and ForensicsHIV detectionHTLV I and II detectionScreening for novel virusesDetection of oncogenic mutationsDiagnosis of retinoblastomaIndividual identificationInstruments and SuppliesOrganizing a laboratory for PCR workLow-cost air-driven cycling ovenBasic equipment and supplies for the PCR lab