This two-volume book is a valuable resource to students, researchers, scientists, commercial producers, consultants and policymakers interested in agriculture or plant sciences particularly in date palm biotechnology. Chapters in Date Palm Biotechnology Protocols: Volume 2: Germplasm Conservation and Molecular Breeding guides readers through methods and protocols on germplasm in vitro conservation, molecular analysis of in vitro cultures, genetic diversity, cultivar identity, gender identification, genomics, and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Date Palm Biotechnology Protocols: Volume 2: Germplasm Conservation and Molecular Breeding aims to supplement the previous volume and to provide precise stepwise protocols in the field of date palm biotechnology.

Includes cutting-edge methods and protocols

Storage and Viability Assessment of Date Palm Pollen.- In Vitro Conservation of Date Palm Tissue Cultures.- Cryopreservation of Date Palm Pro-embryonic Masses Using the D Cryo-Plate Technique.- In Vitro Cryopreservation of Date Palm Caulogenic Meristems.- In Vitro Conservation of Date Palm Shoot-Tip Explants and Callus Cultures under Minimal-Growth Conditions.- In Vitro Conservation of Date Palm Somatic Embryos Using Growth Retardant Conditions.- Encapsulation of Date Palm Somatic Embryos: Synthetic Seeds.- Evaluation of Clonal Fidelity of Micropropagated Date Palm by Random Amplified Polymorphic DNA (RAPD).- Molecular Identification of Fungal Contamination in Date Palm Tissue Cultures.- Genetic Diversity Analysis of Date Palm Using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR).- Date Palm Genetic Diversity Analysis Using Microsatellite Polymorphism.- Assessing Date Palm Genetic Diversity Using Different Molecular Markers.- Molecular Analysis of Date Palm Genetic Diversity Using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR).- Determining Phylogenetic Relationships among Date Palm Cultivars Using Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) Markers.- Genotyping and Molecular Identification of Date Palm Cultivars Using Inter Simple Sequence Repeat (ISSR) Markers.- Molecular Identification of Date Palm Cultivars Using random amplified polymorphic DNA (RAPD) Markers.- Early Sex Identification in Date Palm by Male Specific Sequence-Characterized Amplified Region (SCAR) markers.- Gender Identification in Date Palm Using Molecular Markers. - Development of Sex-Specific PCR-based Markers in Date Palm.- Date Palm Sex Differentiation Based on Fluorescence In Situ Hybridization (FISH).- Characterization and Amplification of Gene-Based Simple Sequence Repeats (SSR) Markers in Date Palm.- Mitochondrial Molecular Markers for Resistance to Bayoud Disease in Date Palm.- Analysis of Expressed Sequence Tags (EST) in Date Palm. - Development of Genomic Simple Sequence Repeats (SSR) by Enrichment Libraries in Date Palm.- MicroRNA Expression in Multistage Date Fruit Development.- Proteome of Abiotic Stress Tolerance in Date Palm.- Proteomics of Development and Germination of Date Palm Zygotic Embryos.- Date Fruit Proteomics during Development and Ripening Stages.