This two-volume book is a valuable resource to students, researchers, scientists, commercial producers, consultants and policymakers interested in agriculture or plant sciences particularly in date palm biotechnology. Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications is comprised of 27 chapters covering adventitious organogenesis, somatic embryogenesis, contamination, hyperhydricity, acclimatization, cell suspension, protoplast and bioreactors, genetic transformation secondary metabolites, and abiotic stress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Date Palm Biotechnology Protocols, Volume 1: Tissue Culture and Applications aims to supplement the previous volume and to provide precise stepwise protocols in the field of date palm biotechnology.

Includes cutting-edge methods and protocols

Cultivar-Dependent Direct Organogenesis of Date Palm from Shoot Tip Explants.- NAA-Induced Direct Organogenesis from Female Immature Inflorescence Explants of Date Palm.- Direct Organogenesis from Immature Female Inflorescence of Date Palm by Gradual Reduction of 2,4-D Concentration.- Optimized Direct Organogenesis from Shoot Tip Explants of Date Palm.- Direct Organogenesis and Indirect Somatic Embryogenesis by In Vitro Reversion of Mature Female Floral Buds to a Vegetative State.- Enhanced Indirect Somatic Embryogenesis of Date Palm Using Low Levels of Seawater.- Enhanced Indirect Somatic Embryogenesis from Shoot-Tip Explants of Date Palm by Gradual Reductions of 2,4-D Concentration.- Indirect Somatic Embryogenesis from Mature Inflorescence Explant of Date Palm.- Indirect Somatic Embryogenesis of Date Palm Using Juvenile Leaf Explants and Low 2,4-D Concentration.- Desiccation-Enhanced Maturation and Germination of Date Palm Somatic Embryos Derived from Cell Suspension Culture.- Desiccation and Cold Hardening of Date Palm Somatic Embryos Improve Germination.- Histological Evidence of Indirect Somatic Embryogenesis from Immature Female Date Palm Inflorescences.- Histological Analysis of Developmental Stages of Direct Somatic Embryogenesis Induced from In Vitro Leaf Explants of Date Palm.- Identifying and Controlling Contamination of Date Palm Tissue Cultures.- Controlling Hyperhydricity in Date Palm In Vitro Culture by Reduced Concentration of Nitrate Nutrients.- Improvement of In Vitro Date Palm Plantlet Acclimatization Rate with Kinetin and Hoagland Solution.- Plant Regeneration from Somatic Embryogenic Suspension Cultures of Date Palm.- Synchronization of Somatic Embryogenesis in Date Palm Suspension Culture Using Abscisic Acid.- Microcalli Induction in Protoplasts Isolated from Embryogenic Callus of Date Palm. - Temporary Immersion System for Date Palm Micropropagation. - Plantform Bioreactor for Mass Micropropagation of Date Palm.- Genetic Transformation of Date Palm via Microprojectile Bombardment.- Microprojectile Bombardment Transformation of Date Palm Using the Insecticidal Cholesterol Oxidase (ChoA) Gene.- Transient GUS Gene Expression in Date Palm Fruit Using Agroinjection Transformation Technique.- Bioreactor Steroid Production and Analysis of Date Palm Embryogenic Callus.- Extraction and Estimation of Secondary Metabolites from Date Palm Cell Suspension Cultures.- In Vitro Assessment of Abiotic Stress in Date Palm: Salinity and Drought.