In Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots (QDs) as fluorescent probes in Life Sciences. While the second section covers important features about QDs¿ preparative processes and characterizations for their successful application as fluorophores. The third part presents main aspects related to QDs methods applied to live cells and tissues. The fourth section focuses on QDs experiments in small animals and the fifth part demonstrates the versatility of QDs in a set of FRET applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Thorough and intuitive, In Quantum Dots: Applications in Biology, Second Edition aids scientists in continuing to study QDs by providing information about methods and protocols helping to expand their research.

Includes cutting-edge methods and protocols

Part I ¿ Introduction to Quantum Dots1. Quantum Dots as Biophotonics ToolsCarlos Lenz Cesar Part II ¿ Quantum Dots¿ Preparative Processes and Characterizations2. Photoligation Combined with Zwitterion-Modified Lipoic Acid Ligands Provides Compact and Biocompatible Quantum DotsNaiqian Zhan, Goutam Palui, Henry Grise, and Hedi Mattoussi 3. Analysis of Quantum Dots and Their Conjugates by Capillary Electrophoresis with Detection of Laser-Induced Luminescence Karel Klepárník, Vladimíra Datinská, Ivona Vorá¿ová, and Marcela Lišková 4. Advanced Procedure for Oriented Conjugation of Full-Size Antibodies with Quantum DotsKristina Brazhnik, Igor Nabiev, and Alyona Sukhanova 5. Quantum Dots-Antibody Conjugates via Carbodiimide-Mediated Coupling for Cellular ImagingDaniel Alistair East, Michael Todd, and Ian James Bruce 6. Measuring the Hydrodynamic Radius of Quantum Dots by Fluorescence Correlation SpectroscopyAndré A de Thomaz, Diogo B. Almeida, and Carlos Lenz Cesar 7. Quantum Dots Fluorescence Quantum Yield Measured by Thermal Lens SpectroscopyCarlos Estupiñán-López, Christian Tolentino Dominguez, Paulo. E. Cabral Filho, Adriana Fontes, Renato E. de Araujo 8. Semiquantitative Fluorescence Method for Bioconjugation AnalysisAluízio G. Brasil Jr., Kilmara H. G. Carvalho, Elisa S. Leite, Adriana Fontes, Beate S. Santos Part III ¿ Quantum Dots for Live Cells and Tissues Applications9. Assembly, Characterization and Delivery of Quantum Dot Labeled Biotinylated Lipid ParticlesValeria Sigot 10. Oriented Conjugation of Single-Domain Antibodies and Quantum DotsKristina Brazhnik, Igor Nabiev, and Alyona Sukhanova 11. Functional Integration of Quantum Dot Labeled Mesenchymal Stem Cells in a Cardiac MicroenvironmentMaria C. Collins, Philip R. Gunst, and Barbara J. Muller-Borer 12. In Vitro Approaches to Assessing the Toxicity of Quantum DotsRyan S. McMahan, Vivian Lee, William C. Parks, Terrance J. Kavanagh, and David L. Eaton 13. Basics for the Preparation of Quantum Dots and their Interactions with Living CellsXiue Jiang, Jing Bai, and Tiantian Wang Part IV ¿ QDs for Small Animals Applications14. In Vivo Approaches to Assessing the Toxicity of Quantum DotsDavid K Scoville, Christopher M Schaupp, Francois Baneyx,  and Terrance J Kavanagh 15. Quantum Dots for Imaging Neural Cells In Vitro and In VivoAngela O. Choi, Kevin D. Neibert, and Dusica Maysinger 16. A Short-Term Inhalation Study Protocol ¿ Designed for Testing of Toxicity and Fate of NanomaterialsLan Ma-Hock, Thomas Hofmann, Robert Landsiedel, and Bennard van Ravenzwaay Part V ¿ Quantum Dots for FRET Applications17. Quantitative Measurement of Proteolytic Rates with Quantum Dot-Peptide Substrate Conjugates and Förster Resonance Energy TransferMiao Wu, Eleonora Petryayeva, Igor L. Medintz, and W. Russ Algar 18. Solid-Phase Supports for the in situ Assembly of Quantum Dot-FRET Hybridization Assays in MicrofluidicsAnthony J. Tavares, M. Omair Noor, Uvaraj Uddayasankar, Ulrich J. Krull, and Charles H. Vannoy