In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes.

Immunohistochemistry Quality Management and Regulation.- Standardization of Diagnostic Immunohistochemistry.- Automated Immunohistochemistry Overview.- Geisinger Immunohistochemical Antibodies and Staining Protocols.- Immunohistochemistry: A Dako Perspective.- Immunohistochemistry: Ventana Perspective.- Rabbit Monoclonal Antibodies.- Detection of Phosphoproteins and Cancer Pathways.- Overview of Predictive Biomarkers and Integration of IHC into Molecular Pathology.- Tissue Microarray.- Unknown Primary/Undifferentiated Neoplasm.- Exfoliative Cytology and Effusions.- Breast.- Predictive Biomarkers in Breast Cancer:  ER, PR and Her-2/neu.- Central Nerve System.- Thyroid, Parathyroid and Adrenal Glands.- Salivary Glands and Head and Neck.- Pleuropulmonary and Mediastinal Neoplasms.- Uterus.- Ovary.- Prostate Gland.- Urinary Bladder and Urachus.- Kidney.- Testis and Paratesticular Tissues.- Pancreas and Ampulla.- Liver, Bile Ducts and Gallbladder.- Upper Gastrointestinal Tract.- Lower Gastrointestinal Tract.- Soft Tissue and Bone Tumors. Lymph Node.- Bone Marrow.- Infectious Diseases.- Skin.- Application of Direct Immunofluorescence for Skin and Mucosal Biopsies: A Practical Review.- Application of Fluorescent in Situ Hybridization (FISH) in Surgical and Cytologic Specimens (Solid Tumors, Hematopoietic Tumors, Urine, Bile Duct Brushing and Bronchoscopy).