Beschreibung:
Cajal and De Castro's Neurohistological Methods provides the first English translation of Fernando de Castro's 1933 publication "Elementos de Técnica Micrográfica del Sistema Nervioso." A student of the famed founder of modern neuroscience, with Santiago Ramon y Cajal also serving as the Editor of the original text, Fernando de Castro recorded all the various protocols that had been used in his laboratory by his students in order to provide a manual of histological procedures specifically designed for the fine structure of the nervous system. This renowned text is virtually unknown in its original form outside the Spanish-speaking world.In a text that reads like a mix between a recipe book and an alchemical manuscript, authors Miguel Merchan, Javier DeFelipe, and Fernando de Castro (descendant of the 1933 publication's author) put the new translation into historical context. This book is also beautifully illustrated with plates of histological techniques, provides a quick guide to new vocabulary, and the author's notes on the translated text. This pivotal work of classic neurohistological techniques is a wonderful addition to the Cajal library.
Principles of Micrographical Techniques for the Nervous SystemDedicationPreliminary InformationList of PlatesSection IHistorical ContextCHAPTER IAuthors and Their Backgrounds by JAIME A. MERCHAN and JORGE PRIETOSection IIBasic Histological TechniquesChapter IIThe Microscope and the FixativesI. MicroscopeII. FixativesFormaldehyde-based FixativesFixatives with Osmic AcidFixatives Based on Hypnotic SubstancesCHAPTER IIIDecalcifying AgentsCHAPTER IVGeneral Dissociation ProceduresDissociationSectioningFrozen Section Procedure (EN 8)Celloidin and Collodion EmbeddingParaffin EmbeddingGelatin EmbeddingSuperficial Paraffin ImbeddingCHAPTER VHandling and Mounting Serial SectionsSections of celloidin-embedded materialSections of paraffin-embedded materialCHAPTER VIGeneral Staining MethodsSome Remarks on the Staining ProcessSources and Composition of DyesCHAPTER VIIFormulae for Carmine and HaematoxylinDouble and Triple StainsModifications of the Iron-Haematoxylin. Methods for the Staining of MitochondriaCHAPTER VIIISection Mounting and PreservationMounting Sections that Require Previous Dehydration and ClarifyingSection Mounting without Dehydration and ClarifyingSection IIISpecial TechniquesCHAPTER IXMethods for the Demonstration of Neuronal MorphologyCHAPTER XContinuation of the Methods for Demonstrating the Morphology of NeuronsFixation, Sectioning and Mounting Techniques for Methylene Blue Stained MaterialCHAPTER XIMethods to Study the Structure of the Nerve CellNissl Chromatic BodiesMethods for Staining the NeurofibrilsSilver Impregnation MethodsImpregnation Of The Golgi ApparatusDemonstration of Plastosomes, Centrosome, Oxidases and Pigmentary SpherulesNucleus and Cell MembraneCHAPTER XIIStaining of Neuronal Axons in the CentresCHAPTER XIIIMyelin StainingCHAPTER XIVColouration of the Macroglia, Microglia and OligodendrogliaMacrogliaDemonstration of the Gliosomes and MitochondriaImpregnation of Oligodendroglia and MicrogliaCHAPTERXVMethods to Demonstrate the Connective TissueMethods For Staining The Connective TissueImpregnation Methods for Connective TissueSupravital Staining with Acidic DyesCHAPTER XVIMethods for the Demonstration of Substances Produced by Alterations of the Cell MetabolismDemonstration of some Cell GranulesDemonstration of Fats and Lipoid SubstancesDemonstration of Ferric PigmentsDemonstration of non-Ferric PigmentsGlycogen DemonstrationDemonstration of Amyloid Substance and Corpora AmylaceaDemonstration of Hyaline DegenerationCalcareous SubstancesCHAPTER XVIIMethods for the Demonstration of the Peripheral Nerves under both Normal and Pathological ConditionsDemonstration of Ranvier NodesMethods to Stain the Schwann CellsMethods for Myelin StainingLantermann Incisures; Spiral Apparatus; Spinous Double Bracelet; Neurokeratin Network (EN 48)Demonstration of the Cylinder-Axis and NeurofibrilsDemonstration of the Connective Tissue of Nerve Trunks (Endoneurium, Perineurium and Epineurium)CHAPTER XVIIIMethods for the Demonstration of Peripheral Nerve EndingsGold Chloride MethodsDemonstration of Nerve Endings with the Golgi MethodDemonstration of Peripheral Nerve Endings with Ehrlich's Methylene Blue ProcedureNeurofibrillary Methods Capable of Demonstrating the Nerve EndingsMethods to Demonstrate the Nerve Trunks and Nerve Endings in Organs Protected by Bone TissueProcedures for Staining the Sympathetic and Dorsal Root GangliaCHAPTERXIXTechniques for the Demonstration of the Nervous Tissue of InvertebratesMethods Capable of Revealing the Morphology of NeuronsMethods for Staining NeurofibrilsCHAPTER XXMethods for Demonstrating some Pathogenic MicroorganismsDemonstration of the Syphilis TreponemaDemonstration of the Trypanosomes in Sleeping SicknessColouration of Negri Bodies in RabiesDemonstration of the Tuberculosis BacillusEditors' NotesVocabulary by the EditorsBibliography